ISOLASI DAN IDENTIFIKASI EUGENOL DARI TANGKAI CENGKEH JENIS ZANZIBAR (Eugenia caryopillata, Thumb)

Abstract

A research was done to isolate and identify eugenol from clove stalk of Zanzibar
variety. Isolation of clove stalk oil was done using steam destilation technique, and for identification
using Gas Chromatography-Mass Spectrophotometer. The isolate from clove stalk oil of Zanzibar
variety, fresh and dried variable has 0,565% and 0,485% yield. The oil was yellow and has cloves
scent. From the test of clove stalk oil quality, fresh and dried variable was known that its water
content were 0,22% and 0,33%., specific weight 1,027 and 1,068 g/mL., with refraction index
1,5186. Beside eugenol, GC-MS analyzes showed that other constituent component in clove stalk oilw
were methyl benzena, methyl salisilat, caryophylin oxide, trans-caryophylin, a-Humulene. Eugenol
can be isolate from clove stalk oil by the addition of NaOH, acidify with HCl and then extracted
using petroleum eter as a solvent. Eugenol that obtained from clove stalk oil, fresh and dried variable
were 66,47 % and 76,92%, respectively. Eugenol analysis from isolated clove stalk, fresh and dried
variable has 90,10% and 93,96% purities, respectively. Eugenol obtained from fractionation was
analyzes using GC, and spiking technique identification.