AKTIVITAS ENTOMOPATOGEN Serratia marcescens Bizio TERHADAP MORTALITAS LARVA KUMBANG KELAPA (Brontispa longissima) Gestro

Siti Ramla S. Kahar, Ani Hasan, Chairunnisa Lamangantjo

Abstract


Entomopathogen is one of the biological agents that infects the insects and can damage the body's metabolic system of insects. This objective of the study were to determine the entomopathogenic activity of S. marcescens on mortality of coconut leaf beetles larvae (B. longissima), and the time needed to reach LT50. The study used an experimental method with 6 treatments of varying S.marcescens volumes, consisting of A (distilled water as a control), B (5 ml), C (7.5 ml), D (10 ml), E (12.5 ml), and F (15 ml) with 4 replications. The data were analyzed using ANOVA and Probit LT50 test. The results showed that the volume of S.marcescens had an effect on mortality of coconut leaf beetle B. longissimi larvae. The best mortality rate at treatment E is 78% with the value of LT50 in treatment F is 42.15 hours. S.marcescens has entomopathogenic activity in coconut leaf beetles (B. longissima) Gestro larvae


Keywords


Entomopatogen, Serratia marcescens, Brontispa longissima, Mortality

Full Text:

PDF

References


Bhargavi, P, L. & RS. Prakasham,. 2012. Proteolytic Enzyme Production by Isolated Serratia sp. RSPB11: Role of Environmental Parameters. Current Trends in Biotechnology and Pharmacy. 6(1): 55-65

Bidari, F., M. Shams-Bakhsh & M. Mehrabadi. 2018. Isolation and Characterization of a Serratia marcescens with Insecticidal Activity from Polyphylla olivieri (Col.: Scarabaeidae). Journal of Applied Entomology. 142(2): 162–172.

Giang, H. T. & S. Nakamura. 2009. The Study on Biological Characteristics of Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae). J. Sci. Dev. 7(2): 159–164.

Herdatiarni, F., T. Himawan & Rachmawaty, R. 2014. Eksplorasi Cendawan Entomopatogen Beauveria sp. Menggunakan Serangga Umpan pada Komoditas Jagung, Tomat dan Wortel Organik di Batu, Malang. Jurnal HPT. 1(3): 1-11

Hidayat, N., MC.Padaga & S. Suhartini. 2006. Mikrobiologi Industri. Edisi 1. Andi Offest. Yogyakarta.

Ishi, K., Tatsuo, A., H. Hiroshi & S. Kazuhisa. 2014. Serratia marcescens Suppresses Host Cellular Immunity via the Production of an Adhesion-Inhibitory Factor against Immunosurveillance Cells. The Journal of Biological Chemistry. 289(9) :5876–5888.

Jumiarti, P. 2012. Pemurnian dan Karakterisasi Protein Insektisidal dari Bakteri Entomopatogen Serratia marcescens. [Skripsi]. Bogor: Institut Pertanian Bogor.

Lumentut, N. & Indrawanto, C. 2013. Biologi Brontispa Longissima Varitas Frogatti , Selebensis dan Javana pada Kelapa Dalam Mapanget dan Kelapa Genjah Raja. B. Palma. 14(2): 76–81.

Lumentut, N., S. Karinda., L. Sulistyowati & RD.Puspitarini. 2013. The Demographic of Brontispa longissima variety of Celebensis Gestro (Coleoptera: Chrysomelidae) on Mapanget Tall Coconut and Brown Dwarf Coconut. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS). 6(2): 33–37.

Muliawan, S. 2007. Bakteri Anaerob yang Erat Kaitannya dengan Problem di Klinik: Diagnosis dan Penatalaksanaan. Edisi 1. Buku Kedokteran EGC. Jakara.

Nestle, M. & WK. Roberts. 1969. An Extracellular Nuclease from Serratia marcescens. The Journal of Biological Chemistry. 244(19): 5219-5225.

Pratiwi, R, S.,TE. Susanto & YAK. Wardani. 2015. Enzim Kitinase dan Aplikasi di Bidang Industri: Kajian Pustaka. Jurnal Pangan dan Agroindustri. 3(3): 878-887.

Priyatno, TP., YA. Dahliani., Y. Suryadi., IM. Samudra., DN. Susilowati., I. Rusmana & C. Irwan. 2011. Identifikasi Entomopatogen Bakteri Merah Pada Wereng Batang Coklat (Nilaparvata lugens Stål.). Jurnal Agrobiogen. 7(2): 85–95

Purkayastha, GD., P. Mangar., A. Saha & D. Saha. 2018. Evaluation of the Biocontrol Efficacy of a Serratia marcescens Strain Indigenous to Tea Rhizosphere for the Management of Root Rot Disease in Tea. Plos One.13(2):1-27.

Rini, M. S., R. Rahardian., M. Hadi & D. Zulfiana. 2016. Uji Efikasi Beberapa Isolat Bakteri Entomopatogen terhadap Kecoak (Orthoptera) Periplaneta americana (L.) dan Blatella germanica (L.) dalam Skala Laboratorium. Jurnal Biologi. 5(2): 1–10.

Salaki, CL. 2011. Eksplorasi Bakteri Entomopatogenik Pengendali Hama Plutella Xylostella dan Spodoptera sp. pada Tanaman Kubis Bunga dan Brokoli. Eugenia. 17(3): 209-219.

Tampubolon, DY., Y. Pangestiningsih., F. Zahara & F. Manik. Uji Patogenitas Bacillus thuringiensis dan Metarhizium anisopliae terhadap Mortalitas Spodoptera litura Fabr (Lepidoptera: Noctuidae) di Laboratorium. Jurnal Online Agroteknologi. 1(3):783-793.

Yus, ID., BT. Rahardjo & T. Himawan. 2014. Pengaruh Aplikasi Pseudomonas fluorescen dan Bacillus subtilis terhadap Mortalitas Nematoda Puru Akar (Meloidogyne javanica) di Laboratorium. Jurnal HPT. 2(3): 9-17.

Wicaksono, S., E. Kusdiyantini & Raharjo, B. 2017. Pertumbuhan dan Produksi Pigmen Merah oleh Serratia marcescens pada Berbagai Sumber Karbon. Jurnal Biologi. 6(3): 66–75.

Widariyanto, R., MI. Pinem & F. Zahara. 2017. Patogenitas Beberapa Cendawan Entomopatogen (Lecanicillium lecanii, Metarhizium anisopliae, dan Beauveria bassiana) terhadap Aphis glycinespadaTanaman Kedelai. Jurnal Agroekoteknologi FP USU. 5(2): 8- 16




DOI: https://doi.org/10.34312/jebj.v1i2.2430

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Siti Ramla S. Kahar, Ani Hasan, Chairunnisa Lamangantjo

Lisensi Creative Commons
Karya ini dilisensikan dengan Lisensi Internasional Creative Commons Attribution-ShareAlike 4.0 .

 

<!--