The performance of Chlorella vulgaris growth on mass-scale cultivation

Dini Alvateha; Siska Falentina; Rarasrum Dyah Kasitowati; Sutianto Pratama Suherman; Luthfiana Aprilianita Sari; Sulastri Arsad

doi:10.37905/tjas.v1i2.8123

The performance of Chlorella vulgaris growth on mass-scale cultivation

Dini Alvateha, Siska Falentina, Rarasrum Dyah Kasitowati, Sutianto Pratama Suherman, Luthfiana Aprilianita Sari, Sulastri Arsad

Abstract

Phytoplankton have many benefits, including as a primary producer, natural food, bioindicator, and water pollution treatment. For this reason, their availability needs to be managed, one of which is through cultivation. The purpose of this study was to analyze the mass scale cultivation of Chlorella vulgaris. The research was conducted at the Technical Implementation Unit of Freshwater and Brackish Water Aquaculture, Situbondo, using a descriptive method. The data were analyzed statistically using MS. Excel 2016 software, and a multiple linear regression test was carried out to determine the effect of water quality parameters on the growth of C. vulgaris using the SPSS 16.0 application. The cultivation process started from strain preparation, water preparation, tank and culture media preparation, inoculation, fertilization, and then harvesting. The initial density of C. vulgaris used was 145x104 Cell. mL-1 in tank 1 and 188x104 Cell. mL-1 in tank 2. The results showed that the cell density value of C. vulgaris increased every day until it entered the exponential phase, namely on the 4th day of the culture activity, which was 507 x 104 in tank 1 and 536 x 104 Cell. mL-1 in tank 2. Furthermore, the value of water quality parameters that affected the growth of C. vulgaris in tank 1 and tank 2 was dissolved oxygen of 4.82-6.97 mg. L-1, pH 8.2-9.1, transparency of 20-45 cm, temperature was 26.8-28.2 oC, nitrate of 0.10-0.50 mg. L-1, phosphate of 0.75-2 mg. L-1, and salinity of 30-39 ppt.

Keywords

Microalgae; Mass scale; Cultivation techniques; Water quality

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References

APHA. (1998). Standard Methods for the Examination of Water and Wastewater. 20th Edn, APHA, AWWA and WPCF, Washington.

Aprilliyanti, S., Soeprobowati, T. R., & Yulianto, B. (2016). Hubungan kemelimpahan Chlorella sp dengan kualitas lingkungan perairan pada skala semi masal di BBPBAP Jepara. Jurnal Ilmu Lingkungan, 14(2), 77-81. https://www.neliti.com/publications/101625/hubungan-kemelimpahan-chlorella-sp-dengan-kualitas-lingkungan-perairan-pada-skal.

Becker, W. (1994). Mikroalgae: Biotechnology and Microbiology. Cambridge University Press (112-146). https://books.google.co.id/books?hl=id&lr=&id=KAKx4I7NWEYC&oi=fnd&pg=PP299&dq=Microalgae:+Biotechnology+and+Microbiology&ots=037tSNOVdN&sig=JebVxAWPB4zD7okEopCkIDSBko&redir_esc=y#v=onepage&q=Microalgae%3A%20Biotechnology%20and%20Microbiology&f=false.

Boroh, R., Litaay, M., Umar, M. R., & Ambeng. (2019). Pertumbuhan Chlorella sp. pada beberapa kombinasi media kultur. Jurnal Biologi Makassar, 4(2), 129-137. https://journal.unhas.ac.id/index.php/bioma/article/view/6759.

Boyd, C. E. (1990). Water Quality in Ponds for Aquaculture. Experiment Station Alabama (482 hlm). https://agris.fao.org/agris-search/search.do?recordID=US19930090347.

Chia, M. A., Lombardi, A. T., & Melao, M. D. G. G. (2013). Growth and biochemical composition of Chlorella vulgaris in different growth media. Anais da Academia Brasileira de Ciencias, 85(4), 1427-1438. https://www.scielo.br/pdf/aabc/v85n4/0001-3765-aabc-00-00-6613.pdf.

Choudhury, A. K., & Pal, R. (2010). Phytoplankton and nutrient dynamics of shallow coastal stations at Bay of Bengal, Eastern Indian coast. Aquatic Ecology, 44, 55-71. https://link.springer.com/article/10.1007/s10452-009-9252-9.

Creswell, L. (2010). Phytoplankton culture for aquaculture feed. Southern Regional Aquaculture Center, 1-13. http://fisheries.tamu.edu/files/2013/09/SRAC-Publication-No.-5004-Phytoplankton-Culture-for-Aquaculture-Feed.pdf.

Daliry, S., Hallajisani, A., Roshandeh, J. M., Nouri, H., & Golzary, A. (2017). Investigation of optimal condition for Chlorella vulgaris microalgae growth. Global Journal of Environmental Science and Management, 3(2), 217-230. https://www.sid.ir/en/journal/ViewPaper.aspx?id=525847.

Effendi, H. (2003). Telaah Kualitas Air bagi Pengelolaan Sumberdaya dan Lingkungan Perairan. Penerbit Kanisius.

Hadiyanto., & Azim, M. (2012). Mikroalga Sumber Pangan dan Energi Masa Depan. Semarang: UPT UNDIP Press. http://eprints.undip.ac.id/48607/1/Buku_Mikroalga___B5.pdf.

Junda, M. (2018). Development of intensive shrimp farming, Litopenaeus vannamei in land-based ponds: production and management. Journal of Physics: Conference Series, 1028, 1-6. https://iopscience.iop.org/article/10.1088/1742-6596/1028/1/012020/meta.

Kandi, I. W. (2018). Teknik kultur fitoplankton Chlorella vulgaris skala laboratorium sebagai pakan alami Daphnia sp. di Balai Pengembangan Teknologi Perikanan Budidaya (BPTPB) Cangkringan, Sleman, Yogyakarta. Universitas Airlangga Repository . http://repository.unair.ac.id/78502/.

Maresi, S. R. P., Priyanti., & Yunita, E. (2015). Fitoplankton sebagai bioindikator saprobitas perairan di Situ Bulakan Kota Tangerang. Al-Kauniyah Jurnal Biologi, 8(2), 113-122. http://journal.uinjkt.ac.id/index.php/kauniyah/article/view/2697.

Marshito, I. (2012). Produktifitas primer dan struktur komunitas pada berbagai substrat perifiton pada berbagai substrat buatan di Sungai Kromong Pacet Mojokerto [Skripsi program studi S-1 Biologi Departemen Biologi, Universitas Airlangga Surabaya]. Universitas Airlangga Repository. http://repository.unair.ac.id/25672/.

Novianti, T., Zainuri, M., & Widowati, I. (2017). Studi tentang pertumbuhan mikroalga Chlorella vulgaris yang dikultivasi berdasarkan sumber cahaya yang berbeda. MANGIFERA EDU: Jurnal Biologi and Pendidikan Biologi, 1(2), 1-8. https://scholar.google.co.id/scholar?hl=id&as_sdt=0%2C5&q=Studi+tentang+pertumbuhan+mikroalga+Chlorella+vulgaris+yang+dikultivasi+berdasarkan+sumber+cahaya+yang+berbeda&btnG=.

Nurlaili, F. R., Hendrawan, Y., & Nugroho, W. A. (2015). Pengaruh dosis penambahan bakteri (Azospirillum sp.) terhadap kelimpahan populasi mikroalga (Chlorella sp.) pada media kultur limbah cair biogas (setelah proses anaerob). Jurnal Keteknikan Pertanian Tropis dan Biosistem, 3(2), 121-126. https://jkptb.ub.ac.id/index.php/jkptb/article/view/265.

Persada, A. Y., Navia, Z. I., Saputri, A., Putri, K. A., & Fajar, B. A. (2019). Inventaris jenis fitoplankton di Pulau Pusong, Langsa, Aceh. Elkawine: Journal of Islamic Science and Technology, 5(1), 67-75. https://core.ac.uk/download/pdf/228448775.pdf.

Prasetio, H., Purwiyanto, A. I. S., & Agussalim, A. 2016. Analisis logam berat timbal (Pb) dan tembaga (Cu) dalam plankton di Muara Banyuasin Provinsi Sumatera Selatan. MASPARI JOURNAL, 8(2), 73-82.

Prayogo, I., & Arifin, M. (2015). Teknik kultur pakan alami Chlorella sp. dan Rotifera sp. skala massal dan manajemen pemberian pakan alami pada larva kerapu cantang. Samakia: Jurnal Ilmu Perikanan, 6(2), 125-134. https://core.ac.uk/download/pdf/276635732.pdf.

Romdhane, F. H., Zheng, X., Jaouen, P., Pruvost, J., Grizeau, D., Croue, J. P., & Bourseau, P. (2013). The culture of Chlorella vulgaris in a recycled supernatant: effects on biomass production and medium quality. Bioresource Technology, 132, 285-292. https://www.sciencedirect.com/science/article/pii/S0960852413000485?casa_token=xBc3bnMDpYAAAAA:EUgSdSszJDDiC_xwYUqMEtdcuiYMn2sfpZEkAUA_DUgC0yaWDXlSNvPL_fdHI0_wrVR-aW_H0Q4.

Rumanti, M., Rudiyanti, S., & Suparjo, M. N. (2014). Hubungan antara kandungan nitrat dan fosfat dengan kelimpahan fitoplankton di Sungai Bremi Kabupaten Pekalongan. DIPONEGORO JOURNAL OF MAQUARES, 3(1), 168-176. https://ejournal3.undip.ac.id/index.php/maquares/article/view/4434.

Salgueiro, J. L., Perez, L., Maceiras, R., Sanchez, A., & Cancela, A. (2016). Bioremediation of wastewater using Chlorella vulgaris microalgae: phosphorus and organic matter. International Journal of Environmental Research, 10(3), 465-470. https://ijer.ut.ac.ir/article_58766.html.

Salman, J. M., Hadi, S. J., & Mutaer, A. A. (2013). Spatial and temporal distribution of phytoplankton and some related physical and chemical properties in Al-Abasia River (Euphrates), Iraq. International Journal of Geology, Earth & Environmental Sciences, 3(3), 155-169. http://www.uobabylon.edu.iq/eprints/publication_1_8546_416.pdf.

Widayat., Philia, J., & Wibisono, J. (2018). Cultivation of microalgae Chlorella sp on fresh water and waste water of tofu industry. E3S Web of Conferences, 31, 04009. https://www.e3s-conferences.org/articles/e3sconf/abs/2018/06/e3sconf_icenis2018_04009/e3sconf_icenis2018_04009.html.

Winder, M., & Sommer, U. (2012). Phytoplankton response to a changing climate. Hydrobiologia, 698, 5-16. https://link.springer.com/article/10.1007/s10750-012-1149-2.

DOI: https://doi.org/10.37905/tjas.v1i2.8123 ';

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