Uji Kualitatif dan Penetapan Kadar dengan Metode Spiking pada Logam Pb di Dalam Minuman Kopi Kaleng

Husnafa Patriani, Fajar Nugraha, Hadi Kurniawan

Abstract


Lead (Pb) is a heavy metal that is naturally present in the earth’s crust. This metal is dispersed in nature in small amounts through natural processes. Lead that accumulates in the environment is not biodegradable and its toxicity does not change. Contamination of lead (Pb) meta; in canned drinks can occur due to imperfect packaging, damage or rust. Lead metal is obtained from soldering tools. This study aims to identify the presence of Pb metal in canned coffee drinks. In addition, to find out the method of preparation that was carried out correctly the spiking method was used. Samples were taken in Pontianak City with purposive sampling technique. The sample of this research is canned coffee drinks with 3 different brands (brands A, B and C) circulating in the city of Pontianak. The qualitative test was carried out using 0.5 N KI reagent and 2 M HCl which was dropped into the sample. A positive result on a sample that was dripped with 0.5 N KI reagent was a yellow precipitate, while a positive result on a sample that was dripped with 2 M HCl reagent was a white precipitate. The spiking method was carried out by adding 0.3 mL of 1000 ppm Pb standard solution and adding the sample to a volume of 2 mL. Then, wet digestion was carried out using 10 mL HNO3 and 2 mL H2O2 as solvent. The results of the qualitative test showed that all samples with 0.5 N KI reagent were clear and black precipitate formed. All samples that were dropped with 2 M HCl reagent were clear and no precipitate was formed. In assay by spiking method, the levels obtained are 73.54 mg/kg and % recovery is 86.667%. All tested samples did not contain Pb metal and the preparation method used was correct.

Keywords


Canned coffee drinks; spiking method; AAS, lead (Pb); qualitative test

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References


. Sunarharum WB, Fibrianto K, Yuwono SS, Nur M. Sains Kopi Indonesia. Malang: UB Press; 2019.

. Badan Pusat Statistik. Badan Pusat Statistik (BPS-Statistics) [Internet]. Badan Pusat Statistika 2021 [cited 2021 Sep 6];Available from: https://www.bps.go.id/indicator/54/132/1/produksi-tanaman-perkebunan.html

. Perdana WW. Analisis Logam Berat Di Kemasan Kaleng. Agroscience (Agsci) 2019;9(2):215–23.

. Dewi. Analisis Campuran Logam Timbal (Pb), Tembaga (Cu), dan Kadmium (Cd) dalam Tepung Gandum secara Spektrofotometri Serapan Atom. Depok: Universitas Indonesia; 2011.

. World Health Organization. Exposure to Lead : A Major Public Health Concern. World Health Organization 2019;1–6.

. BPOM. Badan Pengawas Obat dan Makanan Republik Indonesia No. 20 Tahun 2019 Tentang Kemasan Pangan. Jakarta: 2019.

. Smallman RE, Bishop RJ. Metalurgi Fisik Modern dan Rekayasa Material. Edisi Ke-6. Jakarta: Erlangga; 1999.

. Cahyadi W. Bahaya Pencemaran Timbal pada Makanan dan Minuman. Bandung: Fakultas Teknik Unpas Departemen Farmasi Pascasarjana ITB; 2004.

. Ambarwati NF, Sinaga EM, Gultom E. Analisis tingkat keracunan logam berat Pb pada tukang becak dan pedagang asongan di Jalan Kapten Muslim Medan. Klinikal Sains: Jurnal Analis Kesehatan 2021;9(1):8–14.

. 10. BPOM. Badan Pengawas Obat dan Makanan No. 5 Tahun 2018 Tentang Batas Maksiumum Cemaran Logam dalam Pangan Olahan Biji-bijian. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2018.

. Taufiq M, Kiptiyah K, Muti’ah R. Pengembangan dan validasi prosedur pengukuran logam timbal (Pb) dalam makanan pendamping air susu ibu menggunakan Spektroskopi Serapan Atom. Alchemy: Jurnal Penelitian Kimia 2020;16(1):25–37.

. Roslinda R, Humairah, Zulharmitta. Analisis kadmium (Cd), seng (Zn) dan timbal (Pb) pada susu kental manis kemasan kaleng secara Spektrofotometri Serapan Atom (SSA). Jurnal Farmasi Higea 2013;5(1):62–71.

. Dewi DC. Determinasi kadar logam timbal (Pb) dalam makanan kaleng menggunakan destruksi basah dan destruksi kering. Alchemy: Jurnal Penelitian Kimia 2012;2(1):12–25.

. Harmawan T, Lestari D. Pemeriksaan logam berat cadmium (Cd) dan plumbum (Pb) pada lipstik yang beredar di Pasar Brayan Medan Timur secara Spektrofotometri Serapan Atom (SSA). Quimica: Jurnal Kimia Sains dan Terapan 2020;2(2):18–22.

. Hidayah H, Pratiwi MI, Kusumawati AH, Amal S. Analysis of lead and copper in red grape fruit (Vitis vinifera L.) for sale in Karawang City. Jurnal Ilmiah Farmako Bahari 2021;12(2):122–31.

. Widowaty W, Zakaria A, Nurfiana TY. Analisis cemaran logam (Cu dan Zn) pada kopi bubuk. Agroscience (Agsci) 2020;10(1):79–83.

. Syahriana Y, Desnita R, Luliana S. Verifikasi metode analisis larutan alpha arbutin menggunakan Spektrofotometer UV-Vis Shimadzu UV-2450. Jurnal Untan [Internet] 2019;4(1):1–7. Available from: https://jurnal.untan.ac.id/index.php/jmfarmasi/article/download/36961/75676583553

. Simatupang L. Material Silika Abu Vulkanik Sinabung: Karakteristik dan Aplikasi. Bandung: Media Sains Indonesia; 2021.

. Wulandari EA, Sukesi. Preparasi penentuan kadar logam Pb, Cd dan Cu dalam nugget ayam rumput laut merah (Eucheuma cottonii). Jurnal Sains dan Seni Pomits 2013;2(2):15–7.

. Punčochář M. Chemical Equations [Internet]. Chem. Equations2022;Available from: https://chemequations.com/en/

. Riyanto. Validasi dan Verifikasi Metode Uji. Yogyakarta: Deepublish; 2016.

. Hadi A, Asiah. Statistika Pengendalian Mutu Internal. Bogor: IPB Press; 2018.

. Gandjar IG, Rohman A. Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar; 2017.




DOI: https://doi.org/10.37311/jsscr.v5i1.15802

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