Identifikasi Senyawa Metabolit Sekunder Daun Spigelia anthelmia L. dan Uji Aktifitas Antioksidan Menggunakan Metode DPPH (1,1-Diphenyl-2-Picrylhidrazy)

Muhammad Taupik, A. Mu’thi Andy Suryadi, Jafar La Kilo, Wiwit Zuriati Uno, Samsul Bahri Badjeber

Abstract


Spigelia anthelmia is a type of weed plant that is classified as toxic and is included in the list of herb of medicine. The research aims to determine the secondary metabolite content of Spigelia anthelmia L. leaves extract and antioxidant activity test using the DPPH (1,1-Diphenyl-2-Picrylhydrazy) method. The research samples are extracted by applying the total maceration method with methanol as a solvent and followed by partition method using n-hexane, ethyl acetate, and methanol as solvents. The identification of flavonoid compounds content is performed by employing Thin Layer Chromatography method with methanol as eluent: n-hexane (1:4), ethyl acetate:n-hexane (4:1), ethyl acetate:n-hexane (4:1). Meanwhile, the antioxidant activity test is performed by employing UV-Vis Spectrophotometry method. The result of identification by thin layer chromatography using AlCl3 spray reagent, Lieberman Burchard. Dragendrof, and cerium sulfate, indicate that the Spigelia anthelmia L. leaves extract contains alkaloids, flavonoids, and terpenoids. In addition, the antioxidant activity test of Spigelia anthelmia L. leaves extract is carried out at wavelength of UV 516 nm with an absorbance value of 0,654 A. with a linear equation of y = 0,027 x – 42,83 with a correlation coefficient (R2) = 0,0983 and has the strongest IC50 at the methanol fraction for 7,69 µg/ml.

Keywords


Spigelia anthelmia L.Secondary Metabolite Compounds Thin Layer Chromatography UV-Vis Spectrophotometry

Full Text:

PDF

References


Adikara, I Putu Arya, Dkk. 2013. Studi Histopatologi Hati Tikus Putih (Rattus Novergicus) Yang Diberi Ekstrak Etanol Daun Kedondong (Spondias Dulcis) Secara Oral. Buletin Veteriner Udayana. ISSN: 2085-2495. Vol. 5 No. 2.

Nelson, L.S., Shih, R, D. Balick M.J. & Lampe. K.F., 2007. Handbook Of Poisonous And Injurious Plants, springer.

Duke, J.A. 2010. Handbook Of Medicinal Herb. CRC.

Ditjen POM. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. DepKes RI, Jakarta. Halaman 3-5, 13-17, 30-31.

Martina, R. 2018. Isolasi dan Karakterisasi Senyawa Alkaloid Ekstrak Metanol Sponge Aaptos sp. Bandar Lampung: Universitas Lampung.

Setiawan, I. 2010. Optimasi Ekstrak Cair Cair Fraksi Etanol Daun Dandang Gendis (Clinacanthus mutans). Bandung: IPB.

Sari, Dkk. 2017. Analisis Kualitatif Merkuri Pada Lotion Pemutih Yang Dijual Di Online Shop Daerah Kota Banjarmasin. Jurnal Ilmiah Ibnu Sina. Banjarmasin .Vol 2 No 1. Hal. 13-19.

Harborne, J. B. 1987. Metode Fitokimia. Penuntun Cara Modern Menganalisis Tumbuhan. Terjemahan K. Padmawinata & I. Soediro, Penerbit ITB; Bandung.

Mustafa R., Albadri, Ahmed A., dan Abdulelah, Furqan M. (2019). Nanoemulsion Preparation, Identification and Evaluation Review. Journal of Global Pharma Technology. 196-208

Yusuf, Sumarno, 2010, Pengaruh Penambahan Belimbing Wuluh (Averrhoa bilimbi L.) dan Perebusan terhadap Residu Formalin dan Profil Protein Udang Putih (Letapenaeusa vannamei) Berformalin serta Pemanfaatannya sebagai Sumber Pendidikan Gizi dan Keamanan Pangan pada Masyarakat, Surabaya, Pasca Sarjana FK UB.

Prakash, A. 2001. Antioxidant Activity. Journal Of Analytical Chemsitry. Medallion Laboratories: Analytical Progress. Vol 10, No. 2

Gandjar, I. G. dan Rohman, A. 2007. Kimia Farmasi Analisis. Yogjakarta: Pustaka Pelajar.

Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Fakultas Farmasi: Universitas Indonesia.

Ghosal, M & Mandal, P. 2012. Phytochemical Screening and Antioxidant Activities of Two Selected 'Bihi' Fruits Used as Vegetables in Darjeeling Himalaya. International Journal Of Pharmacy And Pharmaceutical Sciences. ISSN: 0975-1491.

Nurjanah, Izzati, L. & Abdullah, A. 2011. Aktivitas Antioksidan dan Komponen Bioaktif Kerang Pisau (Solen spp). Bogor: Universitas Diponegoro. ISSN: 0853-7291.




DOI: https://doi.org/10.37311/jsscr.v4i3.15927

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Journal Syifa Sciences and Clinical Research

EDITORIAL OFFICE OF JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH
  Department of Pharmacy, Gorontalo State University
Jl. Jenderal Sudirman No.6, Kota Gorontalo, Provinsi Gorontalo 96128, Indonesia
 Email: info.jsscr@ung.ac.id
Google Scholar : JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH

Creative Commons License

Journal Syifa Sciences and Clinical Research (JSSCR) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.