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Isolasi, Karakterisasi, dan Uji Antioksidan Fungi Endofit dari Tanaman Batang Kunyit (Curcuma domestica Val.,)
Abstract
Endophytic fungi live in mutualistic symbiosis and can produce secondary metabolites similar to their host. This research aims to isolate, characterize, and test the antioxidant activity of secondary metabolites from turmeric stems. This research is experimental research where the method used for endophytic fungi isolation is the direct planting method, and antioxidant activity is tested using qualitative and quantitative methods with DPPH (1.1-diphenyl-2-picylhydrazyl). One fungal isolate was obtained with the fungal isolate code (BK). Based on macroscopic and microscopic characterization, the BK fungal isolate indicates the Deuteromycota class's characteristics. The qualitative antioxidant test results indicate that the fraction of BK endophytic fungal isolate indicates antioxidant activity, indicated by the yellow color against a purple background on the TLC plate observed under visible light. The respective ICso values for the ethyl acetate and n-hexane fractions were 52.989 µg/ml and 89.725 µg/ml, categorized as strong antioxidants. The methanol fraction had an ICso value of 119.02 µg/ml, categorized as moderate, while the pure supernatant fraction had an ICso value of 156.86 µg/ml, categorized as weak antioxidant activity.
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. Adefira, E.,(2021).,ՙՙProduk Esktraseluler Isolat Kapang Endofit C.1.1 dan C.3.3 dari Ranting Cempaka Kuning (Michelia champaca L.)sebagai Antimikroba” Jurnal Ilmu Kefarmasian Indonesia , April 2021, hlm. 131-138 Vol. 19, No. 1 ISSN:1693-1831, E-ISSN: 2614-6495.
. Akmalasari, I., Purwati, E. S., & Dewi, R. S. (2013). ՙՙIsolasi dan identifikasi jamur endofit tanaman manggis (Garcinia mangostana L)”. Biosfera, 30(2), 82–89. Antimikroba Ekstrak Etil Asetat Jamur Endofit dari Daun Matoa (Pometia pinnata). Jurnal Farmasi Higea, 12(1).
. Ariyono, R. Q..Djauhari., (2014). ՙՙKeanekaragaman Jamur Endofit Kangkung Darat (Ipomea reptans Poir) pada Lahan Pertanian Organik Konvensional” Jurnal Hama dan Penyakit Tanaman 2 (1) :1-10 ISSN : 2338- 4366.
. Deya Apriliana, Wahyu Widayat, Rolan Rusli.,(2016) “Isolasi Jamur Endofit Rimpang Temu Kunci (Boesenbergia pandurata) Dan Uji Aktivitas Antioksidan”.Penelitian dan Pengembangan Farmaka Tropi Fakultas Farmasi Universitas Mulawarman, Samarinda, Kalimantan Timur,. Hal: 72-77.
. El-Mansi, T, E.M., El-Mansi, E.M.T., Bryce, C.F.A., Demain, A.L., dan Allman, A.R., 2012.”Fermentation Microbiology and Biotechnology”,Third Edition, 3 edition. ed. CRC Press, Boca Raton, FL.
. Fajrina, A., Bakhtra, D. D. A., Mawarni, A. E., & . 2020. Isolasi dan Uji Aktivitas Antimikroba Ekstrak Etil Asetat Jamur Endofit dari Daun Matoa (Pometia pinnata). Jurnal Farmasi Higea, 12(1)
. Kanifah, U., M. Lutfi dan B. Susilo. 2015. Karakterisasi Ekstrak Daun Sirih Merah (Piper Crocatum)Dengan Metode Ekstraksi Non-Thermal Berbantukan Ultrasonik (Kajian Perbandingan Jenis Pelarut Dan Lama Ekstraksi). Jurnal Bioproses Komoditas Tropis. Volume 3 (1) : 73-79.
. Khomsan.,2014. Pangan dan Gizi untuk Kesehatan. Jakarta: PT Raja Grafindo Persada.
. Komala, Oom. 2015. Uji Efektivitas Esktrak Etanol Daun Lidah Mertua (Sansevieria trifasciata Prain) Terhadap Khamir Candida albican. Universitas Pakuan: Bogor.
. Munawaroh, S. 2012. "Ekstraksi Minyak Daun Jeruk Purut (Citrus hystrixD.C.) Dengan Pelarut Etanol dan N-Heksana".Jurnal Teknik Kimia. Universitas Negeri Semarang:Semarang, Vol. 2, No.1.
. Pakaya, M. S., Uno, W. Z., Papeo, D. R. P., & Moo, D. R. 2022. Isolasi dan Karakterisasi Jamur Endofit Lamun (Thalassia hemprichii) Dari Kawasan Teluk Tomini. Journal Syifa Sciences and Clinical Research, 4(3).
. Priharta. (2018). “Isolasi, Karakterisasi dan Uji Aktifitas Antibiotik Bakteri Endofit dari Tumbuhan Eceng Gondok (Eichornia crassipes) di Danau Maninjau, Sumatera Barat”. Jurnal Farmasi. 4(2): 41-50.
. Prakash, A., (2014), Antioxidant Activity, Medallion Laboratories Analytical Progress, vol. 19, No.2.
. Reihani dan Oddershede. 2016. Screening Minyak Nabati Untuk Minyak Imersi Mikroskop Optik. Institut Teknologi Sepuluh Nopember: Surabaya
. Rianto, A., Isrul, M., Anggarini, S., & Saleh, A. (2018). Isolasi Dan Identifikasi Fungi Endofit Daun Jambu Mete (Anacardium occidentale L.) Sebagai Antibakteri Terhadap Salmonella typhimurium. Jurnal Mandala Pharmacon Indonesia, 4(02), 109–121
. Ristiyani, E. (2015). Perbandingan Efektifitas Antibakteri Ekstrak Maserasi Dan Soxhletasi Daun Pandan Wangi (Pandanus amaryllifolius Roxb.) Terhadap Bakteri Bacillus subtillis. Politeknik Harapan Bersama, Tegal.
. Rizka Dwi Widya Putri, Nuniek Herdyastuti.(2021),Potensi Senyawa Antioksidan Yang Dihasilkan Bakteri Endofit Pada Daun Jambu Biji (Psidium guajava L.), Departement of Chemistry, Faculty of Matematics and Natural Sciences, Universitas Negeri Surabaya
. Salmia, S. (2016). Analisis Kadar Flavonoid Total Ekstrak Kulit Batang Kedondong Bangkok (Spondias Dulcis) dengan Metode Spektrofotometri Uv-Vis. Thesis. [Skripsi]. Universitas Islam Negeri Alauddin Makassar. Hal 48.
. Siburian., Elfrida,T.,(2012).,Pengaruh Suhu dan Waktu Penyimpanan Terhadap Pertumbuhan Bakteri dan Fungi Ikan Bandeng., Biologi Unnes Journal Of Life Science.ISSN 2252-6277 Hal: 101:105.
. Wahdaningsih, S., Wahyuono, S., & Styowati, E.P., (2013) : Isolation And Identification Of Antioxidant Compounds In Fern Stems (Alsophila Glauca J.Sm) Using Dpph Method (2,2-Diphenyl-1-Picrylhydrazyl). Department Of Biology, Faculty Of Pharmacy , Universitas Gadjah Mada, Yogyakarta, Indonesia ; ISSN : 1410-5918.
DOI: https://doi.org/10.37311/jsscr.v5i2.20341
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