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Efektivitas Fraksi Metabolit Sekunder Jamur Endofit Lamun (Thalassia hemprichii) dari Kawasan Teluk Tomini Sebagai Antibakteri Jerawat
Abstract
Endophytic fungi live in their host tissues, so that endophytic fungi can produce secondary metabolites that are similar to their hosts as well. In this case, the use of endophytic fungi can reduce the exploitation of seagrass populations (Thalassia hemprichii) in Tomini Bay. This study aims to examine the effectiveness of the secondary metabolite fraction of the endophytic seagrass fungus (Thalassia hemprichii) from Tomini Bay against acne-causing bacteria. The test method used is disc diffusion method (Kirby Bauer), by measuring the inhibition zones of the ethanol fraction, ethyl acetate fraction, and the N-hexan fraction of the secondary metabolite fraction of endophytic fungi against acne-causing bacteria. The liquid-liquid partition results of the secondary metabolites of seagrass endophytic fungi from the Tomini Bay area were the N-Hexan fraction of 3.06 grams, the Chloroform fraction of 2.90 grams, and the Ethyl Acetate fraction of 1.53 grams. While the effectiveness test of the N-Hexan, Chloroform, and Ethyl Acetate fractions of secondary metabolites of endophytic seagrass microbes from the Tomini Bay area showed that the N-Hexan fraction at a concentration of 75% had greater inhibition on each of the acne-causing bacteria, namely Propionibacterium acne 17 .33 mm (strong category), Staphylococcus aureus 17.86 mm (strong category), and Staphylococcus epidermidis 16.01 mm (strong category).
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N. Z. Hendrawan, “Formulasi dan uji aktivitas antibakteri gel Nanosilver terhadap bakteri Staphylococcus aureus secara in vitro.,” Universitas Islam Negeri Maulana Malik Ibrahim, malang, 2018.
R. G. Bramantio dan Dr. Devi Usdiana Rosyidah, M. Sc, “Uji Efektivitas Antibakteri Minyak Atsiri Daun Sirih Merah (Piper Crocatum Ruiz & Pav.) Terhadap Staphylococcus epidermidis secara In Vitro,” Universitas Muhammadiyah Surakarta, surakarta, 2018.
E. R. Taminggu dan T. Tahril, “Identifikasi Senyawa Metabolit Sekunder Pada Batang dan Daun Lamun (Seagrass) di Teluk Palu,” Media Eksakta, vol. 18, no. 1, hlm. 6–11, Mei 2022.
G. V. B. Ulina, - Sumardianto, dan - Romadhon, “Potensi Antibakteri Ekstrak Lamun Thalassia Hemprichii Pada Fillet Ikan Lele (Clarias batracus) Selama Penyimpanan Dingin,” J. Pengolah. Dan Bioteknol. Has. Perikan., vol. 5, no. 1, Art. no. 1, Jan 2016.
W. Z. RX Tan, “Endophytes: A Rich Source Of Functional Metabolites,” Nat. Prod. Rep., vol. 18(4), hlm. 448–459, 2001.
M. S. Pakaya, “Isolasi dan Karakterisasi Jamur Endofit Lamun (Thalassia hemprichii) Dari Kawasan Teluk Tomini”.
A. Amirullah, S. Sartini, dan F. Nainu, “Fungi Endofit dari Tanaman Secang (Caesalpinia sappan L) Sebagai Penghasil Senyawa Antioksidan: Endophytic Fungi from Secang (Caesalpinia sappan L) as Producer of Antioxidant Compounds,” J. Farm. Galen. Galen. J. Pharm. E-J., vol. 5, no. 1, hlm. 26–32, Mar 2019.
K. Noor, I. S. M dan Febriyanti, R, “Pengaruh Perbedaan Pelarut Dan Uji Sifat Fisik Sediaan Krim Pada Ekstrak Kulit Bawang Merah (Allium cepa L).,” J. Ilm. Farm., 2020.
A. Rendowaty, A. Djamaan, dan D. Handayani, “Waktu Kultivasi Optimal dan Aktivitas Antibakteri dari Ekstrak Etil Asetat Jamur Simbion Aspergillus unguis (WR8) dengan Haliclona fascigera,” J. Sains Farm. Klin., vol. 4, no. 1, hlm. 49, Des 2017.
H. A. Aprilia, D. Pringgenies, dan E. Yudiati, “Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius Artemia sp,” J. Mar. Res., vol. 1, no. 1, Art. no. 1, 2012.
K. Kusnadi dan E. T. Devi, “Isolasi Dan Identifikasi Senyawa Flavanoid Pada Ekstrak Daun Seledri (Apium Graveolens L.) Dengan Metode Refluks,” PSEJ Pancasakti Sci. Educ. J., vol. 2, no. 1, Apr 2017.
B. O. I. Kinam, W. C. Prabowo, S. Supriatno, dan R. Rusli, “Skrining Fitokimia dan Profil KLT Ekstrak dan Fraksi dari Daun Berenuk (Cresentia cujete L.) serta Uji DPPH: Phytochemical Screening and TLC Profile of Extracts and Fractions from Leaves of Berenuk (Cresentia cujete L.) and DPPH Test,” Proceeding Mulawarman Pharm. Conf., vol. 14, hlm. 339–347, Des 2021.
S. Nurayni dan D. Handayani, “Optimization Of Andalas Endophytic Fungi Fermentation Conditions (Morus Macroura Miq.) Isolate CED 3 To Produce Antibacterial Compounds”.
C. K. Brooks GF, M. S. Butel JS, dan Mietzner, T.A, Mikrobiologi Kedokteran Jawetz, Melnick, & Adelberg, 25 ed. jakarta: buku kedokteran EGC, 2013.
M. Agustina, L. Soegianto, dan R. Sinansari, “Uji Aktivitas Antibakteri Hasil Fermentasi Kulit Buah Naga Merah (Hylocereus polyrhizus) terhadap Propionibacterium acnes,” J PHARM SCI, vol. 8, no. 1, 2021.
DOI: https://doi.org/10.37311/jsscr.v6i1.21457
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