Deteksi Bakteri Salmonella sp. dengan Kultur Darah Pada Pasien Widal Positif di Laboratorium Klinik X

Nurul Istiqomah, Novia Agustina, Salsa Bellamilenia Putri

Abstract


Until now, the gold standard test for diagnosing typhoid fever is a bacterial culture or also known as a gall culture examination. Blood cultures have the best sensitivity (40–60%) when performed in the first-early second week. Purpose: this study aims to identify the presence of Salmonella sp. in the blood of Widal positive patients. Method: this research is a descriptive study with a cross-sectional approach. This study used the blood culture method. The media used in this study were oxgall, SSA, KIA and IMViC media. The results showed that 20% of Salmonella typhi bacteria were found, 7% of other bacteria and 73% of bacteria did not grow on the media. The macroscopic characteristics of salmonella bacteria found were round colonies, clear in color, convex surface, flat colony edges, semi mucoid consistency, no reduction of tellurit. The microscopic characteristics are rod-shaped, spread arrangement, red in color and are Gram negative. Cultivation on IMViC media showed Indole -, MR +, VP -, Citrate -, KIA medium alkaline slope, acid base, H2S +, gas -. Conclusion: Salmonella thypi was found in the blood cultures of positive widal patients.

Keywords


Gall culture; Typoid fever; Salmonella sp.; Widal test

Full Text:

PDF

References


. Rijal S. Analisis Metode Serologi Widal Lapangan, Widal Pembanding, Dan Kultur Pada Penderita Suspek Demam Tifoid Di Sulawesi Selatan. Jurnal Ilmiah As-Syifaa . 2014;6(1):43–55.

. Sucipta MAA. Baku Emas Pemeriksaan Laboratorium Demam Tifoid Pada Anak. Jurnal Skala Husada . 2015 Apr;12(1):22–6.

. Setiana, Putri G, Kautsar AP. Perbandingan Metode Diagnosis Demam Tifoid. Farmaka. 2016;14(1):94–103.

. Arfamaini R. Rekomendasi IDAI mengenai Pemeriksaan Penunjang Diagnostik Demam Tifoid. Applied Microbiology and Biotechnology . 2016;85(1):71–9.

. Sri D., Langkah S., Widya A., Wayan TA. KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK. Biologi, Sains, Lingkungan, dan Pembelajarannya dalam Upaya Peningkatan Daya Saing Bangsa. 2012;496–501.

. Marhani N. Identifikasi Salmonella Typhi Pada Penderita Demam Tifoid Di Puskesmas Malili. Voice of Midwifery . 2018;8(1):34–43.

. Amarantini C. Seleksi Bakteri Salmonella Typhi Dari Kultur Darah Penderita Demam Tifoid. Prosiding Seminar Nasional Penelitian, Pendidikan Dan Penerapan MIPA, Fakultas MIPA, Universitas Negeri Yogyakarta . 2016 May;13–20.

. Levin KA. Study Design III: Cross-Sectional Studies. Evid Based Dent. 2006;7(1):24–5.

. Notoatmodjo S. Metodologi Penelitian Kesehatan. Jakarta: RinekaCipta; 2010.

. Cappucino JG, Sherman N. Microbiology, A Laboratory Manual Tenth Edition. United Statesof America: Pearson Education, Inc; 2014.

. Lestari IDAMD. Identifikasi dan Diagnosis Infeksi Bakteri Salmonella thypi. [Denpasar]: Universitas Udayana; 2017.

. Ulya, Nanda Najmatul, Inayah Fitri, Devis Ika Widyawati. Gambaran Makroskopis Dan Mikroskopis Bakteri Salmonella Typhi Dan Salmonella Paratyphi Pada Penderita Demam Tifoid. Jurnal Sintesis . 2020 Dec;1(2):40–6.

. Bergeys DH, Boone DR. Bergey’s Manual of Systematic Bacteriology. In: Book of Microbiology. 3rd ed. New York: Springer Science-Business Media; 2005.

. Susi AR, Muhammad HG. Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia Coli. Indonesian Journal of Pharmaceutical Science and Technology. 2017 Jun;4(2):50–6.

. Mogasale V, Ramani E, Mogasale VV, Park J. What proportion of Salmonella Typhi cases are detected by blood culture: A systematic literature review. Ann Clin Microbiol Antimicrob. 2016 May;15(32):1–8.

. Gunawan AP, Djuminar A, Ernawati, Chaidir L. Pengembangan prekultur oxgall sebagai sampel klinis untuk deteksi Salmonella typhi dengan metode real-time PCR. Jurnal Teknologi Laboratorium. 2018 Sep;7(2):70–7.




DOI: https://doi.org/10.37311/jsscr.v5i2.21497

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Journal Syifa Sciences and Clinical Research

EDITORIAL OFFICE OF JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH
  Department of Pharmacy, Gorontalo State University
Jl. Jenderal Sudirman No.6, Kota Gorontalo, Provinsi Gorontalo 96128, Indonesia
 Email: info.jsscr@ung.ac.id
Google Scholar : JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH

Creative Commons License

Journal Syifa Sciences and Clinical Research (JSSCR) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.