Uji Antioksidan Limbah Kulit Nanas (Ananas comosus L.) Menggunakan Metode 2,2-Difenil-1-Pikrilhidrazil (DPPH)

Hamsidar Hasan, Muhammad Taupik, A. Mu'thi Andy Suryadi, Mohamad Aprianto Paneo, Shofiyah Badjeber

Abstract


Pineapple (Ananas comosus L.) is a plant that has the potential as a source of bioactive compounds for antioxidants. The flavonoid content in this plant contains an antioxidant mechanism by inhibiting the initiation process of radical compounds, so that the oxidation process inhibited and prevents oxidative stress in the body. This research aimed to identify secondary metabolites in pineapple peel waste and the potential antioxidant activity of the ethyl acetate fraction of pineapple peel. This research began with an extraction process using a multilevel maceration method with three different solvents encompassing n-hexane, ethyl acetate, and methanol. Each fraction was subjected to a phytochemical screening test, thin layer chromatography test, and antioxidant test qualitatively by spraying 0,2% DPPH. Subsequently, the positive fraction in the qualitative test was continued with the quantitative test using a UV-Vis spectrophotometer. As a result, the secondary metabolites detected in pineapple peel fraction were alkaloid, flavonoid, steroid, and tannin. Meanwhile, the potential antioxidant activity was the ethyl acetate fraction of pineapple peel with an IC50 value of 40,52 ppm which was included in the very strong antioxidant category


Keywords


Phytochemical Screening; Pineapple Peel; Antioxidant; UV-Vis Spectrophotometer

Full Text:

PDF

References


Andina, Lisa & Musfirah, Y. Total Phenolic Content of Cortex and Leaves of Ramania (Bouea Macrophylla Griffith) and Antioxidant Activity Assay by DPPH Method. Journal of Pharmaceutical, Biological and Chemical Sciences. 2017. ISSN: 0975-8585: Banjarbaru

Arista, N., & Siregar, R. M. Uji Aktivitas Antioksidan Ekstrak Kulit Pisang Barangan (Musa acuminata Linn) dengan Metode DPPH. Nautical: Jurnal Ilmiah Multidisiplin Indonesia. 2023. 1(12), 1477-1484

Auwal, M.S., Saka, S., Mairiga, I.A., Sanda, K.A., Shuaibu, A. & Ibrahim, A. Preliminary Phytochemical and Elemental Analysis of Aqueous And Fractionated Pod Extracts Of Acacia Nilotica (Thorn mimosa). Vet Res forum an Int Q J. 2014. 5(2), 95–100

Bariyyah, Sitti K. Uji Aktivitas Antioksidan Terhadap DPPH dan Identifikasi Golongan Senyawa Aktif Ekstrak Kasar Miroalga (Chlorella sp.) Hasil Kultivasi dalam Medium Ekstrak Tauge. 2013. UIN Maulana Malik Ibrahim: Malang

Fajriaty, I., Hariyanto, I.H., Saputra, I.R., & Silitonga, M. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Dari Ekstrak Etanol Buah Lerak (Sapindus rarak). Jurnal Pendidikan Informatika dan Sains. 2017. 6(2), 243

Forestryana, D. & Arnida. Phytochemical Screenings and Thin Layer Chromatography Analysis of Ethanol Extract Jeruju Leaf (Hydrolea spinosa L.). Jurnal Ilmiah Farmako Bahari. 2020. 11(2), 113-124

George, V. C., Kumar, D. N., Suresh, P. K., & Kumar, R. A. Antioxidant, DNA Protective Efficacy and HPLC analysis of Annona muricata (Soursop) Extracts. Journal of Food Science and Technology. 2015. 52, 2328-2335

Hanani, E. Analisis Fitokimia. ECG. Penerbit Buku Kedokteran ECG. 2015. Jakarta.

Hasan, H. Skrining Fitokimia dan Uji Aktivitas Antioksidan Jantung Pisang Goroho (Musa acuminafe L.) dengan Metode 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). Indonesian Journal of Pharmaceutical Education. 2021. 1(3), 136-141

Indarto, I., Narulita, W., Anggoro, B. S., & Novitasari, A. Aktivitas Antibakteri Ekstrak Daun Binahong Terhadap Propionibacterium acnes. Biosfer: Jurnal Tadris Biologi. 2019. 10(1), 67-78

Juariah, S., Irawan, M.P., & Yuliana. Efektifitas Ekstrak Etanol Kulit Nanas (Ananas Comosus L. Merr) terhadap Trichophyton mentaghrophytes. Journal of Pharmacy and Science. 2018. 1(2), 1-9

Latief, M., Tafzi, F., & Saputra, A. Aktivitas Antioksidan Ekstrak Metanol Beberapa Bagian Tanaman Kayu Manis (Cinnamomum burmani) Asal Kabupaten Kerinci Provinsi Jambi. Prosiding Semirata FMIPA Universitas Lampung. 2013

Lung, J. K. S., & Destiani, D. P. Uji Aktivitas Antioksidan Vitamin A, C, E dengan Metode DPPH. Farmaka. 2017. 15(1), 53-62

Molyneux, P. The Use of the Stable Free Radical Diphenylpicryl-Hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin Journal of Science and Technology. 2004. 26, 11–19

Mustaqimah. Identifikasi Senyawa Flavonoid Ekstrak Etanol Daun Karinat dengan Metode Klt. Jurnal Sains Medisina. 2023. 1(1), 169-171

Prameswari, O. M., & Widjanarko, S. B. Uji Efek Ekstrak Air Daun Pandan Wangi Terhadap Penurunan Kadar Glukosa Darah Dan Histopatologi Tikus Diabetes Mellitus [In Press 2014]. Jurnal Pangan dan Agroindustri. 2014. 2(2), 16-27

Punbasayakul, N., Samart, K., & Sudmee, W. Antimicrobial Activity of Pineapple Peel Extract. Proceeding of Innovation of Functional Foods in Asia Conference. 2018. Phayao. Thailand. Thailand:IFFA.

Rohmaniyah, M. Uji Antioksidan Ekatrak Etanol 80% dan Fraksi Aktif Rumput Bambu (Lopaherum gracileBrongn) Menggunakan Metode DPPH Serta Identifikasi Senyawa Aktifnya. Skripsi. 2016. Jurusan Kimia. Fakultas Sains dan Teknologi. Universitas Islam Negeri Maulana Malik Ibrahim. Malang.

Sasmita, S.O., Purwanti, L., & Sadiyah, E.R. Perbandingan Aktivitas Antioksidan Ekstrak Etanol Daun, Kulit Buah dan Biji Kopi Arabika (Coffea arabica L.) dengan Metode Peredaman Radikal Bebas DPPH. Prosiding Farmasi. 2014. 5(2), 699-705

Sayuti, K. & Yenrina, R. Antioksidan Alami dan Sintetik. 2015. Andalas University Press. Padang

Sutomo, Wahyuono, S., Rianto, S., & Setyowati, E.P. Isolation and Identification of Actives Compound of N-Hexane Fraction from Kasturi (Mangifera casturi Kosterm.) Against Antioxidant and Immunomodulatory Activity. J. Biol. Sci. 2013. 13(7), 596–604

Sutomo, Wahyuono, S., Rianto, S., Setyowati, E.P., & Yuswanto, A. Antioxidant Activity Assay of Extracts and Active Fractions of Kasturi Fruit (Mangifera casturi Kosterm.) Using 1,1-diphenyl-2-picrylhydrazyl Method. J. Nat. Prod. 2014. (7), 124–130

Ukieyanna, E. Aktivitas antioksidan, kadar fenolik, dan flavanoid total tumbuhan suruhan (Peperomia pellucid L. Kunth). 2012. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor

Yuhernita & Juniarti. Analisa Senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian




DOI: https://doi.org/10.37311/jsscr.v5i3.21573

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Journal Syifa Sciences and Clinical Research

EDITORIAL OFFICE OF JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH
  Department of Pharmacy, Gorontalo State University
Jl. Jenderal Sudirman No.6, Kota Gorontalo, Provinsi Gorontalo 96128, Indonesia
 Email: info.jsscr@ung.ac.id
Google Scholar : JOURNAL SYIFA SCIENCES AND CLINICAL RESEARCH

Creative Commons License

Journal Syifa Sciences and Clinical Research (JSSCR) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.