Antiproliferative Activity of Borassus flabellifer L. Seed Coat Extracts on Oral Carcinoma Cells

Pretty Trisfilha; Janti Sudiono; Komariah Komariah; Celine Chelsea Hartono; Namita Namita Prayorina Nugroho; Raynita Rosalinda Angeline

doi:10.37311/jsscr.v7i2.31570

Antiproliferative Activity of Borassus flabellifer L. Seed Coat Extracts on Oral Carcinoma Cells

Pretty Trisfilha, Janti Sudiono, Komariah Komariah, Celine Chelsea Hartono, Namita Namita Prayorina Nugroho, Raynita Rosalinda Angeline

Abstract

Borassus flabellifer L. is a plant in Arecaceae family, widely distributed and cultivated in tropical Asian countries. Different parts of Borassus flabellifer L., including the fruit, seeds, leaves, and roots, are used in traditional medicine due to their rich bioactive compound content. The present study aims to investigate the in vitro anti-proliferative activity of EBSC on HSC-3 cells. The extract were prepared using an ethanolic solvent, and its phytochemical composition were analyzed to identify potential bioactive compounds. The antiproliferative effects of the extract were evaluated using the Cell Counting Kit-8 (CCK-8) assay. The phytochemical analysis revealed the presence of phenolic, flavonoid, terpenoid, tannin, and alkaloid in EBSC. In CCK-8 assay, EBSC exhibited dose-dependent antiproliferative effects on HSC-3 cells. Significant inhibition of cell proliferation was observed at 750 μg/mL, comparable to doxorubicin (3 μM, p < 0.001), following 24, 72, and 120 hours of treatment. EBSC shows promising potential as a natural therapeutic candidate for OSCC treatment and warrants further in vivo studies.

Keywords

Borassus flabellifer L.; Seed Coat Extract; Antiproliferative; OSCC; Natural Anticancer Agent; CCK-8 Assay

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References

G. Bai et al., “Geniposide inhibits cell proliferation and migration in human oral squamous carcinoma cells via AMPK and JNK signaling pathways,” Exp. Ther. Med., vol. 24, no. 6, p. 706, Oct. 2022. [Online]. Available: https://doi.org/10.3892/etm.2022.11642

V. Bouvard et al., “IARC Perspective on Oral Cancer Prevention,” N. Engl. J. Med., vol. 387, no. 21, pp. 1999–2005, Nov. 2022. [Online]. Available: https://doi.org/10.1056/NEJMsr2210097

N. Xu et al., “Inhibition of human oral squamous cell carcinoma proliferation and migration by prodrug-activating suicide gene therapies,” Exp. Ther. Med., vol. 25, no. 2, p. 92, Jan. 2023. [Online]. Available: https://doi.org/10.3892/etm.2023.11790

A. D. Nazhvani, N. Sarafraz, F. Askari, F. Heidari, and M. Razmkhah, “Anti-Cancer Effects of Traditional Medicinal Herbs on Oral Squamous Cell Carcinoma,” Asian Pac. J. Cancer Prev., vol. 21, no. 2, pp. 479–484, Feb. 2020. [Online]. Available: https://doi.org/10.31557/APJCP.2020.21.2.479

K.-W. Chang et al., “Aqueous Extract of Sparganii Rhizoma and Curcumae Rhizoma Induces Apoptosis and Inhibits Migration in Human Oral Squamous Cell Carcinoma,” J. Food Biochem., vol. 2023, May 2023. [Online]. Available: https://doi.org/10.1155/2023/6564124

P. Tunit, P. Thammarat, S. Okonogi, and C. Chittasupho, “Hydrogel Containing Borassus flabellifer L. Male Flower Extract for Antioxidant, Antimicrobial, and Anti-Inflammatory Activity,” Gels, vol. 8, no. 2, p. 126, Feb. 2022. [Online]. Available: https://doi.org/10.3390/gels8020126

G. Babu and J. J., “Assessment of Anticancer Activity of Ethanolic Extract of Borassus flabellifer Linn. Leaf Against Ehrlich Ascites Carcinoma and Daltons Ascites Lymphoma in Swiss Albino Mice,” Int. Res. J. Pharm., vol. 10, no. 1, pp. 113–118, Jan. 2019. [Online]. Available: https://doi.org/10.7897/2230-8407.100120

G. R. Duddukuri, Y. N. Sastry, and K. K. Chaitanya, “Preliminary Studies on In Vitro Anti-Tumor Activity of Tender Seed Coat Extract of Borassus flabellifer L. On HeLa Cell Line,” Int. J. Curr. Res., vol. 3, no. 7, pp. 075–077, 2011. [Online]. Available: http://www.journalcra.com/?q=node/271

S. M. Banu, N. Vigasini, and S. Surenderan, “In Vitro Antibacterial, Anticancer and Antidiabetic Potential of Freeze-dried Aqueous Borassus flabellifer L. Seed Powder Extract,” Indian J. Pharm. Sci., vol. 84, no. 3, pp. 586–592, 2022. [Online]. Available: https://doi.org/10.36468/pharmaceutical-sciences.964

S. Abirami et al., “In Vitro Antimicrobial, Antiproliferative Activity of Aqueous Extract From Endosperm of Germinated Palmyra Palm Seed (Borassus flabellifer L),” J. Pharm. Negat. Results, vol. 13, no. 7, 2022. [Online]. Available: https://doi.org/10.47750/pnr.2022.13.S07.054

I. Isnaini et al., “Antioxidant and Antiproliferative Activities of Methanol Extract from Melaleuca cajuputi subsp. cumingiana [Turcz.] Fruit,” J. Berk. Ilm. Sains Dan Terap. Kim., vol. 17, no. 1, p. 21, Feb. 2023. [Online]. Available: https://doi.org/10.20527/jstk.v17i1.13055

S. Warnakulasuriya, “Global epidemiology of oral and oropharyngeal cancer,” Oral Oncol., vol. 45, no. 4–5, pp. 309–316, Apr. 2009. [Online]. Available: https://doi.org/10.1016/j.oraloncology.2008.06.002

G. Pannone et al., “The role of human papillomavirus in the pathogenesis of head & neck squamous cell carcinoma: an overview,” Infect. Agent. Cancer, vol. 6, no. 1, p. 4, Dec. 2011. [Online]. Available: https://doi.org/10.1186/1750-9378-6-4

A. Jemal et al., “Global cancer statistics,” CA. Cancer J. Clin., vol. 61, no. 2, pp. 69–90, Mar. 2011. [Online]. Available: https://doi.org/10.3322/caac.20107

F. E.-Z. Amrati et al., “Phytochemical Study on Antioxidant and Antiproliferative Activities of Moroccan Caralluma europaea Extract and Its Bioactive Compound Classes,” Evid. Based Complement. Alternat. Med., vol. 2020, p. 8409718, Jan. 2020. [Online]. Available: https://doi.org/10.1155/2020/8409718

S. Man, W. Gao, Y. Zhang, L. Huang, and C. Liu, “Chemical study and medical application of saponins as anti-cancer agents,” Fitoterapia, vol. 81, no. 7, pp. 703–714, Oct. 2010. [Online]. Available: https://doi.org/10.1016/j.fitote.2010.06.004

Y.-Z. Zhao et al., “Advances in the antitumor activities and mechanisms of action of steroidal saponins,” Chin. J. Nat. Med., vol. 16, no. 10, pp. 732–748, Oct. 2018. [Online]. Available: https://doi.org/10.1016/S1875-5364(18)30113-4

S. H. Kim et al., “Geraniol induces cooperative interaction of apoptosis and autophagy to elicit cell death in PC-3 prostate cancer cells,” Int. J. Oncol., vol. 40, pp. 1683–1690, Dec. 2011. [Online]. Available: https://doi.org/10.3892/ijo.2011.1318

F. Ahmad, K. M. Surapaneni, B. Kamaraj, and B. Kamaraj, “Anti-proliferative activity and apoptotic induction of tannins extracted from Quercus infectoria on oral cancer KB cell lines,” J. Appl. Pharm. Sci., 2024. [Online]. Available: https://doi.org/10.7324/JAPS.2024.174009

DOI: https://doi.org/10.37311/jsscr.v7i2.31570

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