Antibacterial Activity of Matoa (Pometia pinnata) Leaf Extracts and Fractions Against Staphylococcus aureus ATCC 6538P

Nurul Azmi Susilawati; Nadia Ayuwanda S. Akune; M. Fathurrachman Mantali

doi:10.37311/jsscr.v7i3.32370

Antibacterial Activity of Matoa (Pometia pinnata) Leaf Extracts and Fractions Against Staphylococcus aureus ATCC 6538P

Nurul Azmi Susilawati, Nadia Ayuwanda S. Akune, M. Fathurrachman Mantali

Abstract

Matoa (Pometia pinnata) leaves contain alkaloids, flavonoids, tannins, saponins, phenols, steroids, and terpenoids that can act as antibacterial agents. This experimental study aimed to evaluate the antibacterial activity of 70% ethanolic extract and the water, n-hexane, and ethyl acetate fractions of matoa leaves against Staphylococcus aureus ATCC 6538P. Antibacterial testing was performed using the well diffusion method at concentrations of 10%, 20%, and 30% (w/v), prepared in 10% dimethyl sulfoxide (DMSO). Ciprofloxacin 1 µg/well and 10% DMSO were used as positive and negative controls, respectively. Inhibition zones were measured after 24 h incubation at 37 °C in triplicate. The 30% ethyl acetate fraction produced the largest inhibition zone (13.2 mm), followed by the 30% ethanolic extract (11.6 mm), whereas the n-hexane and water fractions yielded smaller zones (9.3 mm and 5.9 mm, respectively, at 30%). Ciprofloxacin produced a very strong inhibition zone of 33.0 mm, while 10% DMSO showed no inhibition. One-way ANOVA with Bonferroni post-hoc analysis indicated that increasing concentration significantly enhanced the inhibition zone for several test materials (p < 0.05). Overall, the 30% ethyl acetate fraction exhibited the strongest in-vitro antibacterial activity among all matoa leaf preparations tested, although its effect remained weaker than that of ciprofloxacin.

Keywords

Staphylococcus aureus; Pometia pinnata; Matoa Leaves; Well diffusion; Antibacterial activity

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DOI: https://doi.org/10.37311/jsscr.v7i3.32370

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