Acute Toxicity and Antioxidant Activity of Ethanol Extracts of Teak Leaves (Tectona grandis L.)

Parawansah Parawansah; Nuralifah Nuralifah; Waode Marianti; Muhammad Syahrul

doi:10.37311/jsscr.v8i1.35256

Acute Toxicity and Antioxidant Activity of Ethanol Extracts of Teak Leaves (Tectona grandis L.)

Parawansah Parawansah, Nuralifah Nuralifah, Waode Marianti, Muhammad Syahrul

Abstract

Toxicity is the ability of a substance to cause damage that affects organisms, so toxicity tests can be used to determine the dosage for human safety. Natural antioxidants can protect the body against damage caused by reactive oxygen compounds, inhibit disease, and inhibit lipid peroxidation in food. Teak leaves (Tectona grandis L.) are thought to have antioxidant potential because they contain flavonoids. This study was conducted to determine the toxicity of teak leaf (Tectona grandis L.) ethanol extract using the Brine Shrimp Lethality Test (BSLT) method and to determine the antioxidant activity of teak leaf (Tectona grandis L.) ethanol extract using the Ferric Reducing Antioxidant Power (FRAP) method. The extract was obtained by maceration using 96% ethanol solvent for 3×24 hours and concentrated at 40°C. The results of The BSLT assay showed a dose-dependent mortality pattern, and probit analysis yielded an LC₅₀ value of 1049.78 µg/mL (95% CI: 11.54–95,483.20 µg/mL), indicating low lethality under in-vitro screening conditions. In the FRAP assay, the extract exhibited ferric-reducing capacity, with increasing absorbance across concentrations of 10–300 µg/mL (mean ± SD: 0.2923–0.4727). The conclusion of this study is The ethanol extract of teak leaves showed low lethality in the BSLT assay and exhibited ferric-reducing capacity in the FRAP assay under in-vitro conditions.

Keywords

Antioxidant; Toxicity; BSLT; FRAP; Tectona grandis L.

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References

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DOI: https://doi.org/10.37311/jsscr.v8i1.35256

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