Efektivitas Daun Bandotan (Ageratum conyzoides L.) terhadap Pendarahan (Bleeding Time) pada Mencit Jantan (Mus musculus)

Abstract

Bandotan (Ageratum conyzoides L) is a plant that functions as an external hemostatic agent.  Bleeding time is the time when bleeding begins to form a platelet plug and vasoconstriction of blood vessels so that blood stops flowing. This study aims to determine the effectiveness and concentration of the ethanol extract of Ageratum conyzoides L leaves in stopping bleeding on male mice through an experimental research. The method employed was the Duke Method. Ageratum conyzoides L leaf extraction was carried out by maceration using 96% ethanol solvent then tested by phytochemical screening. The screening result was tested positive for flavonoids and tannins. The tested animals used were 25 mice which were divided into five groups. Group 1 was given aquadest, group 2, 1 mg / 1 ml of epinephrine drug, group 3, 10% ethanol extract of Ageratum conyzoides leaves, group 4, 20% Ageratum conyzoides leaf ethanol extract, and group 5 was given 40% ethanol extract. Then the data were analyzed using the ANNOVA statistical test (p <0.01). The results of the analysis showed a significant difference in the bleeding stop time of each treatment group and continued with the LSD (Least Significant Different) test. The best concentration of Ageratum conyzoides leaf extract that has the best effectiveness in stopping bleeding was a concentration of 40%.