Konversi Asam Amino L-Valin menjadi Asam Asam 2-hidroksi-3-metilbutirat

Suleman Duengo, Ace Tatang Hidayat, Weny J.A Musa, Rani Maharani

Abstract


The conversion of the amino acid L-valine to 2-hydroxy-3-methylbutyrate has been successfully carried out through a diazotization reaction. This study was conducted to address the limited availability of starting materials or precursors used in the synthesis of depsipeptide compounds. The diazotization reaction of L-Valine was performed using sodium nitrite salt and dilute sulfuric acid. The reaction was carried out at a temperature of 0°C for 2 hours, followed by reflux at room temperature for 15 hours. The synthetic product has a specific optical rotation value, namely 〖[α]〗_D^20 = +8,80. The synthesized product was characterized using IR and HR-TOF MS. The IR spectrum showed absorption characteristics of the carboxyl group, with a broad absorption band for the O-H stretch at υmax 3416.289 cm-1, C-H sp3 stretch at υmax 2965.595 cm-1, and a strong and sharp C=O stretch at υmax 1704.143 cm-1. The formation of 2-hydroxy-3-methylbutyrate in this study was confirmed by the appearance of a molecular ion peak [M+H]+ at m/z 117.0555, which corresponds to the calculated mass of 2-hydroxy-3-methylbutyrate, m/z 117.0552.

Keywords


L-Valine; Conversion; Diazotization; Starting material

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DOI: https://doi.org/10.37905/je.v20i2.37546

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