BIOMASSA MIKROBA DAN AKTIVITASNYA PADA SEDIMEN DAN AIR DANAU LIMBOTO DENGAN TEKNIK PENGAYAAN EX-SITU MIKROKOSMOS

Abstract

The objectives of this study were to count the microbial biomass on sediment and water of Limboto lake and to determinate the microbial diversity on sediment and water based on their
metabolic activity through the enrichment ex-situ microcosmos technique. The study done in 2 steps were sampling of sediment and water on Limboto lake and laboratorium observation. pH and
temperature were physical indicator of sample and laboratory observation was microbial count (bacteria) based on plate count method. The microbial diversity determinated by the enrichment ex-situ
microcosmos technique (winogradky colomn). The microbial diversity observed in 21 days base on the physical and chemistry changing. The study was succesfully counting microbial biomass on sediment
and water Limboto lake. The count of microbial biomass was 6.45x107 CFUgr-1 (in sediment) and 2.18x106 CFUml-1 (the water). The enrichment ex-situ microcosmos technique with winogradsky
colomn was succesfully determinate microbial diversity based on microbial metabolic activity. The microbial metaolic activity was show through physical, chemistry and biological change on sediment
and water sample. That means was aquatic ecosystem on Limboto lake contain some microbial metabolic groups (guilds). The guilds were show microbial activity in the ecosystem, especially on
sediment colomn, microaerophylic and water colomn.