EFEK ASAM METOKSIASETAT (MAA) TERHADAP KUALITAS EMBRIO MENCIT (Mus musculus) SWISS WEBSTER TAHAP PRAIMPLANTASI

Abstract

The effects of methoxyacetic acid on embrio development had been studied. Female mice were mated in the afternoon and the presence of vaginal plug next morning was determined as
day 0 of pregnany. On the day 1 of pregnancy, female mice of treated groups were adminintered with a dose of MAA 2 mmol/kg b.w. by gavage, whereas the control groups were administered with serilized
ditilled water with the same volume. On the day 3 of pregnancy, pregnan mice from treated groups and control groups were sacrificed by cervical dislocation and dissected. Observations were done on the
number of 8 cell embryos, uncompacted morule, compacted morulae, early bastocyst, late blastocyst and abnormal embryos. Observation of number of blastomers was done in day 3.5 of pregnancy using
Tarkowskys\' method. The results showed that single dose of MAA 2 mmol/kg b.w. decreased the percentage of embryos that reached late blastocyst significantly (P<0.01). This was mainly caused by
the increasing of retarded embryos. Besides, the number of abnormal embryos was increased significantly (P<0.05). The decreasing quality of embryos was also shown the decresing of blastomers
significantly (P<0.01).