Antioxidant Activity and Inhibition of Α-Glucosidase from Yellow Root Extract (Fatuoa Pilosa Gaudich) In Vitro

Maria Magdalena Nona Motu Tukan, Samsul Falah, Dimas Andrianto, Najmah Najmah

Abstract


Yellow root (Fatoua pilosa Gaudich) is a type of plant which is found on lowland mostly in Japan, China and usually grow in the dry bushes. Traditionally, the yellow root (Fatuoa pilosa Gaudich) is used to treat the disease of diabetes mellitus and as antiinflamation. One of the causes of diabetes disease is oxidative stress. Oxidative stress can be reduced by using antioxidants. This research aims to explore antioxidant activity and inhibition of α-glucosidase enzyme from yellow root extract (Fatoua pilosa Gaudich.). Quantitative determination of total phenolic by using folin-ciocalteu method declared as gallic acid equivalents (GAE) per gram of extract, levels of total flavonoids with AlCl3 method declared as Quercetin equivalen (QE), and antioxidant activity in fertilization with the DPPH (2, 2-diphenyl-1- picrylhydrazyl) stated in the IC50 (concentration inhibition). The Results showed that the highest levels of total flavonoids was in extract ethanol 96% 199.44 g QE/g extract, while the total phenolic levels highest in 70% ethanol extracts 211.54 mg GAE/g extract. The highest IC50 obtained from antioxidant test of yellow root extrat was 70% ethanol extract 14.62 μg/mL and the most active fraction that can inhibit α-glucosidase enzyme was of ethyl acetate fraction with IC50 values 680.54 μg/mL.

Keywords


Yellow root; Alpha-glucosidase; antioxidants

Full Text:

PDF

References


AG, H. B. B. (1994). Pharmacology of α‐glucosidase inhibition. European Journal of Clinical Investigation, 24(3 S), 3–10. https://doi.org/10.1111/j.1365-2362.1994.tb02249.x

Badarinath, A. V, Rao, K. M., Madhu, C., Chetty, S., Ramkanth, S., Rajan, T. V. S., & Gnanaprakash, K. (2010). A Review on In-vitro Antioxidant Methods: Comparisions, Correlations and Considerations. In International Journal of PharmTech Research CODEN (Vol. 2, Issue 2).

Bisswanger, Hans. (2008). Enzyme kinetics : principles and methods. Wiley-VCH.

Chiang, C.-C., Cheng, M.-J., Peng, C.-F., Huang, H.-Y., & Chen, I.-S. (2010). A Novel Dimeric Coumarin Analog and Antimycobacterial Constituents from Fatoua pilosa.

CHUGH, C. A., MEHTA, S., & DUA, H. (2012). Phytochemical Screening and Evaluation of Biological Activities of Some Medicinal Plants of Phagwara, Punjab †. https://www.researchgate.net/publication/234079737

Farnsworth, N. R. (1966). Biological and Phytochemical Screening of Plants. In JOURNAL O F Pharmaceutical Sciences M ~ T c ~ (Vol. 55, Issue 3).

Hanani, E., Munim, A., & Sekarini, R. (2005). IDENTIFIKASI SENYAWA ANTIOKSIDAN DALAM SPONS CALLYSPONGIA SP DARI KEPULAUAN SERIBU. Majalah Ilmu Kefarmasian, 2(3), 127–133. https://doi.org/10.7454/psr.v2i3.3389

Hasim, H., Andrianto, D., Lestari, E. D., & Faridah, D. N. (2017). Aktivitas antioksidan ekstrak sulur buah naga putih (Hylocereus undatus) dengan metode DPPH dan Rancimat. Jurnal Gizi Dan Pangan, 12(3), 203–210. https://doi.org/10.25182/jgp.2017.12.3.203-210

Javanmardi, J., Stushnoff, C., Locke, E., & Vivanco, J. M. (2003). Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry, 83(4), 547–550. https://doi.org/10.1016/S0308-8146(03)00151-1

Kadifkova Panovska, T., Kulevanova, S., & Stefova, M. (2005). In vitro antioxidant activity of some Teucrium species (Lamiaceae).

Kim, K. Y., Nam, K. A., Kurihara, H., & Kim, S. M. (2008). Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry, 69(16), 2820–2825. https://doi.org/10.1016/j.phytochem.2008.09.007

Seo, S.-Y., Sancheti, S., & Sancheti, S. (2009). Chaenomeles Sinensis: A Potent α-and β-Glucosidase Inhibitor. American Journal of Pharmacology and Toxicology, 4(1), 8–11.

Standar Nasional Indonesia. (1992). Cara Uji Makanan dan Minuman. Badan Standar Nasional .

Stankovic, M. S., Niciforovic, N., Topuzovic, M., & Solujic, S. (2011). Total phenolic content, flavonoid concentrations and antioxidant activity, of the whole plant and plant parts extracts from Teucrium montanum L. var. montanum, f. supinum (L.) reichenb. Biotechnology and Biotechnological Equipment, 25(1), 2222–2227. https://doi.org/10.5504/bbeq.2011.0020

Sugiwati, S., Setiasih, S., & Efy Afifah, dan. (2009). ANTIHYPERGLYCEMIC ACTIVITY OF THE MAHKOTA DEWA [Phaleria macrocarpa (Scheff.) Boerl.] LEAF EXTRACTS AS AN ALPHA-GLUCOSIDASE INHIBITOR (Vol. 13, Issue 2).

Teh, S. S., El-Din Bekhit, A., & Birch, J. (2014). Antioxidative polyphenols from defatted oilseed cakes: Effect of solvents. Antioxidants, 3(1), 67–80. https://doi.org/10.3390/antiox3010067

Vongsak, B., Sithisarn, P., Mangmool, S., Thongpraditchote, S., Wongkrajang, Y., & Gritsanapan, W. (2013). Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Industrial Crops and Products, 44, 566–571. https://doi.org/10.1016/j.indcrop.2012.09.021

Yoshino, M., & Murakami, K. (2009). A graphical method for determining inhibition constants. Journal of Enzyme Inhibition and Medicinal Chemistry, 24(6), 1288–1290. https://doi.org/10.3109/14756360902829766

Zhang, H., Wang, G., Beta, T., & Dong, J. (2015). Inhibitory properties of aqueous ethanol extracts of propolis on alpha-glucosidase. Evidence-Based Complementary and Alternative Medicine, 2015. https://doi.org/10.1155/2015/587383




DOI: https://doi.org/10.34312/jambchem.v5i2.20503

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Jambura Journal of Chemistry



EDITORIAL OFFICE

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.