EFFECTIVENESS OF YOUNG ARECA NUT ON THE GROWTH OF VIBRIO CHOLERAE AND ESCHERICHIA COLI BACTERIA

Deborah Angelin Purba, Juli Elisabeth Mayen Meliala, Juli elisabeth mayen, Hanna Christsela Purba, Erika Yolanda Ginting, Ricky Kinarta Sembiring, Edy Fachrial

Abstract


Diarrhea is caused by impaired intestinal absorption of food and beverages (such as carbohydrates, fats, and proteins) contaminated with bacteria such as Vibrio cholerae and Escherichia coli.  The purpose of this study was to determine the effect of giving young areca nut seeds and what percentage of the concentration had a very good effect on overcoming the growth of Vibrio cholerae and Escherichia coli bacteria. To determine the effect of giving young areca nut and what percentage of concentration has the best effect on overcoming bacterial growth of Vibrio cholerae and Escherichia coli bacteria. The sample used is 5kg. Type of research is True Experimental and the research design is Post-Test Only Control Design the data in this study will be analyzed using SPSS Version 27 software. The concentration of the extract was made into 4 concentrations, namely 20%, 40%, 60%, 80%, and positive control (Ciprofloxacin). Furthermore, the inhibition test was carried out on the two bacteria, namely Vibrio cholerae and Escherichia coli were conducted using NA (Nutrient Agar) media. Research shows that extracts from young areca nut (Arecha catechu L.) seeds can inhibit the growth of both bacteria at high concentrations 20%, 40%, 60%, 80%, and positive control (Ciprofloxacin) with an average diameter of the inhibitory zone test for Vibrio cholera were 13.3 mm, 11.5 mm, 13.4 mm, 13.8 mm and 21.3 mm. And for the power test, the inhibition zones of Escherichia coli were 12.8 mm, 11.5 mm, 11.9 mm, 11.9 mm, 19,4 mm. This study showed that the extract (Arecha Catechu L.) affected the growth effectiveness of Vibrio cholerae and Escherichia coli bacteria from 4 concentrations, namely 20%, 40%, 60%, and 80%.  The effect of young areca nut (Arecha Catechu L.) on bacteria was highest at a concentration of 60%.



Keywords


Cholerae; Diarrhea; Escherichia coli; Vibrio cholera ; Young areca nut

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References


Kemenkes RI. Data Dan Informasi Profil Kesehatan Indonesia 2018. 2019;

Riskesdas. Laporan Nasional RISKESDAS 2018. 2018;

Kemenkes RI. Profil Kesehatan Indonesia Tahun 2019. 2020;

WHO. Cholera. 2021.

Guli MM. Patogenesis Penyakit Kolera Pada Manusia. Biocelebes. 2016;10(2).

Guntina RK. Deteksi Bakteri Vibrio Cholerae. Farmaka. 2017;15(1):93–104.

WHO. E.Coli. 2018.

Lim, J. Y., Yoon, J., & Hovde CJ. A Brief Overview Of Escherichia Coli O157:H7 And Its Plasmid O157. 2013.

Cleveland O. E. Coli Infection. 2021.

Azima F, Putera P, Zulfani R, Hernando R. Rancang Bangun Mesin Pengiris Buah Pinang Muda Tipe Horizontal. Agroteknika. 2018;1(2):63–76.

Wael Mu, Dewi Ss, Maharani Etw. Daya Hambat Infusa Biji Pinang (Areca Catechu L.) Terhadap Bakteri Staphylococcus Aureus. In: Prosiding Seminar Nasional & Internasional. 2017.

Baiti M, Elfrida S, Lipinwati L. Pengaruh Pemberian Ekstrak Ethanol Biji Buah Pinang (Areca Catechu L.) Terhadap Pertumbuhan Staphylococus Aureus Secara In Vitro. JAMBI Med JOURNAL" J Kedokt Dan Kesehatan". 2018;6(1):10–9.

WHO. Cholera. World Health Organization. 2021.

Rxlist. Betel Nut. 2021.

Irwan I, Akuba M. Uji Efektifitas Kombinasi Perasan Jeruk Nipis Dan Mentimun. Jambura J Heal Sci Res. 2019;1(2):71–8.

Jam N, Hajimohammadi R, Gharbani P, Mehrizad A. Evaluation Of Antibacterial Activity Of Aqueous, Ethanolic And Methanolic Extracts Of Areca Nut Fruit On Selected Bacteria. Biomed Res Int. 2021 Apr;2021:6663399.

Bowers MT. How Dangerous Is Betel Nut? 2018.

E. P. Senyawa Bioaktif Tanaman Sereh (Cymbopogon Nardus) Ekstrak Kloroform Dan Etanol Serta Pengaruhnya Terhadap Mikroorganisme Penyebab Diare. Jur Pendidik Biol Fak Pendidik Biol Dan Ilmu Pendidikan Univ Muhammadiyah Malang. 2007;

Purwanti E. Senyawa Bioaktif Tanaman Sereh (Cymbopogon Nardus) Ekstrak Kloroform Dan Etanol Serta Pengaruhnya Terhadap Mikroorganisme Penyebab Diare. Jur Pendidik Biol Fak Pendidik Biol Dan Ilmu Pendidikan Univ Muhammadiyah Malang. 2007;




DOI: https://doi.org/10.35971/jjhsr.v4i3.12164

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