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THE EFFECT OF GREEN TEA EXTRACT ON THE NUMBER OF SECRETORIC EPITELEL CELLS AND EXPRESSION OF ESTROGEN- α RECEPTORS OF WISTAR FALLOPY TUBES EXPOSED TO MSG
Abstract
Stres oksidatif dapat menyebabkan infertilitas melalui berbagai mekanisme, yaitu melalui ketidakseimbangan antioksidan dan radikal bebas, sehingga meningkatkan kerusakan seluler yang disebabkan oleh Reactive oxygen species (ROS). Monosodium glutamat (MSG) adalah garam natrium glutamat, yang digunakan sebagai bahan tambahan makanan sebagai penguat cita rasa. Pemberian MSG tikus secara oral dapat menyebabkan peningkatan Reactive Oxygen Species (ROS) sehingga terjadi stress oksidatif pada jaringan yang memiliki reseptor glutamat. MSG berpengaruh pada anatomi dan fungsi tuba yaitu jumlah sel epitel sekretorik dan ekspresi reseptor estrogen-α. Tujuan penelitian ini adalah untuk membuktikan pengaruh ekstrak teh hijau terhadap jumlah sel epitel sekretorik dan ekspresi RE-α tuba fallopi tikus wistar yang dipapar MSG. Kebaruan penelitian ini adalah menberikan informasi dampak Monosodium Glutamat (MSG) pada manusia. Penelitian ini dilakukan selama 30 hari dan terdiri dari kelompok perlakuan yaitu K(-), K(+) MSG 0,7 mg/gBB, P1 MSG 0,7 mg/BB+ekstrak teh hijau 0,7 mg/tks/hr, P2 MSG 0,7 mg/gBB+ekstrak teh hijau 1,4 mg/tks/hr, P3 MSG 0,7 mg/gBB+ekstrak teh hijau 2,8 mg/tks/hr. Hasil analisa statistik menunjukkan hubungan yang signifikan bahwa pemberian ekstrak teh hijau dosis 0,7 mg dapat meningkatkan jumlah sel epitel sekretorik dan ekspresi RE-α tuba fallopi tikus wistar yang dipapar MSG (p=0,000, R2=50% dan, p=0,000, R2=70,2% ). Kesimpulan adalah membuktikan bahwa ekstrak teh hijau dapat meningkatan jumlah sel epitel sekretorik dan ekspresi RE-α tuba fallopi tikus wistar yang dipapar MSG.
Kata kunci : Ekspresi RE-Α; Ekstrak Teh Hijau; Jumlah Sel Epitel Sekretorik; MSG.
Abstract
Oxidative stress can cause infertility through various mechanisms, namely through antioxidant imbalances and free radicals, thus increasing cellular damage caused by Reactive oxygen species (ROS). Monosodium glutamate (MSG) is a sodium glutamate salt, commonly used as a food additive as a flavor enhancer to affect the nature of food. MSG giving oral mice can cause increased Reactive Oxygen Species (ROS), resulting in oxidative stress in tissues that have glutamate receptors. MSG affects tubal anatomy and function, namely the number of secretory epithelial cells and the expression of estrogen-α receptors. The aim of this study was to prove the effect of green tea extract on the number of secretory epithelial cells and the expression of RE-α fallopian tubes of MSG exposed wistar rats. The novelty of this research is to provide information on the impact of Monosodium Glutamate (MSG) on humans. This research was conducted for 30 days and consisted of treatment group that is K (-), K (+) MSG 0,7 mg/gWB, PI MSG 0,7 mg/gWB+green tea extract 0,7 mg/rat/day, PII MSG 0,7 mg/gWB+green tea extract 1.4 mg/rat/day , PIII MSG 0,7 mg/gWB+green tea extract 2,8 mg/rat/day. The results of statistical analysis showed a significant association that giving 0.7 mg/rat/day of green tea extract increased the number of secretory epithelial cells and the expression of RE-α fallopian tubes of MSG exposed wistar rats (p=0.000, R2=50% and, p=0.000, R2=70.2%). The conclusion of this study is to prove that green tea extract can increase the number of secretory epithelial cells and expression of RE-α fallopian tubes of MSG exposed wistar rats.
Keywords: Green Tea Extract; MSG; Number Of Secretory Epithelial Cells; RE-Α Expression.
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Gupta S, Sekhon L, Aziz N, Agarwal A. The impact of oxidative stress on female reproduction and art: An evidence-based review. Infertil Assist Reprod. 2008;629–42.
Legoh C, Kaseke MM, Pasiak TF. Gambaran histologik hati tikus Wistar yang diberi jus tomat setelah diinduksi monosodium gluatamat. J e-Biomedik. 2017;5(1):2–5.
Harwell B. Biochemistry of oxidative stress. Biochem Soc Trans. 2007;35(5):1147–50.
Limón-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutat Res - Genet Toxicol Environ Mutagen. 2009;674(1–2):137–47.
Shinde A, Ganu J, Naik P. Effect of Free Radicals & Antioxidants on Oxidative Stress: A Review. J Dent Allied Sci. 2012;1(2):63.
Agarwal A, Aponte-Mellado A, Premkumar B, Shaman A, Gupya S. The Effects of Oxidative Stress on Female Reproduction: A Review. Reprod Biol Endocrinol. 2012;10(1):1–31.
BPOM. Peraturan Badan Pengawas Obat Dan Makanan tentang Bahan Tambahan Pangan. Badan pengawas obat dan makanan republik Indones. 2019;1–10.
Ismail N. Assessment of DNA damage in testes from young Wistar male rat treated with monosodium glutamate. Life Sci J. 2012;9(1):930–9.
Walker R, Lupien JR. The Safety Evaluation of Monosodium Glutamate. J Nutr. 2000;130(4S):1049S-52S.
Gill SS, Pulido OM. Review Article: GlutamatE Receptors in Peripheral Tissues: Current Knowledge, Future Research, and Implications for Toxicology. Toxicol Pathol. 2001;29(2):208–23.
Ardyanto T. MSG dan Kesehatan : Sejarah, Efek dan Kontroversinya. Inovasi. 2004;1(XVI):52–6.
Onaolapo OJ, Onaolapo AY, Akanmu MA, Gbola O. Evidence of alterations in brain structure and antioxidant status following ‘low-dose’ monosodium glutamate ingestion. Pathophysiology [Internet]. 2016;23(3):147–56. Available from: http://dx.doi.org/10.1016/j.pathophys.2016.05.001
Matute C, Domercq M, Sánchez-Gómez MV. Glutamate-mediated glial injury: Mechanisms and clinical importance. Glia. 2006;53(2):212–24.
Rueda CB, Llorente-Folch I, Traba J, Amigo I, Gonzalez-Sanchez P, Contreras L, et al. Glutamate excitotoxicity and Ca2+-regulation of respiration: Role of the Ca2+ activated mitochondrial transporters (CaMCs). Biochim Biophys Acta - Bioenerg [Internet]. 2016;1857(8):1158–66. Available from: http://dx.doi.org/10.1016/j.bbabio.2016.04.003
El-Beltagy AE-FBM, Elghaweet HA. Adverse effects of monosodium glutamate on the reproductive organs of adult Female albino rats and the possible ameliorated role of carob (Ceratonia Siliqua). J Biosci Appl Res. 2016;2(3):170–84.
Lyons RA, Saridogan E, Djahanbakhch O. The reproductive significance of human Fallopian tube cilia. Hum Reprod Update. 2006;12(4):363–72.
El-Mowafi, M D, Fallopian T. Reproductive Endocrinology and Infertility. United Stated America: Wayne State University; 2012.
Boot R. Guyton and Hall Textbook of Medical Physiology. 13ed. University of Missisipi Medical Center; 2014. 1039–1046 p.
Parker G, Picut C. Atlas of Histology of the Juvenile Rat. WIL Research, a Charles River Company, Hillsborough, NC, United State. 2016. 203–226 p.
Mosbah R, Yousef MI, Mantovani A. Nicotine-induced reproductive toxicity, oxidative damage, histological changes and haematotoxicity in male rats: The protective effects of green tea extract. Exp Toxicol Pathol [Internet]. 2015;67(3):253–9. Available from: http://dx.doi.org/10.1016/j.etp.2015.01.001
Karlina Y. Siklus estrus dan struktur histologis ovarium tikus putih (. Univ Sebel Maret. 2003;7(April):47–52.
Farombi EO, Onyema OO. Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: Modulatory role of vitamin C, vitamin E and quercetin. Hum Exp Toxicol. 2006;25(5):251–9.
Bylander A. Progesterone’s effect on gamete transport in the fallopian tube. 2014. 63 p.
Umami R, Winarsih S, Studi P, Kebidanan M, Brawijaya U, Mikrobiologi L, et al. Pengaruh Vitamin C dan E terhadap Histologi Tuba Fallopii pada Tikus yang Dipapar MSG The Effect of Vitamin C and E on the Fallopian Tubes Histology of MSG-exposed Rats. J Kedokt Brawijaya. 2014;28(2):63–7.
Mohammed Soliman MEM, Ali A, El-Seify G, El Haroun H. Effect of monosodium glutamate on the ovaries of adult female albino rats and the possible protective role of green tea. Menoufia Med J. 2014;27(4):793.
Afriza D. The Effect of Mercury Vapor and the Role of Green Tea Extract on Brain Cells. J Dent Indones. 2013;20(2):39–45.
Basu A, Betts NM, Mulugeta A, Tong C, Newman E, Lyons TJ. Green tea supplementation increases glutathione and plasma antioxidant capacity in adults with the metabolic syndrome. Nutr Res [Internet]. 2013;33(3):180–7. Available from: http://dx.doi.org/10.1016/j.nutres.2012.12.010
Satoh K, Sakamoto Y, Ogata A, Nagai F, Mikuriya H, Numazawa M, et al. Inhibition of aromatase activity by green tea extract catechins and their endocrinological effects of oral administration in rats. Food Chem Toxicol. 2002;40(7):925–33.
Frei B, Higdon J V. Proceedings of the Third International Scientific Symposium on Tea and Human Health : Role of Flavonoids in the Diet. Antioxidant Activity of Tea Polyphenols In Vivo: Evidence from Animal Studies. J Nutr. 2003;(February):3285–92.
Rashidinejad A, Birch EJ, Everett DW. Antioxidant activity and recovery of green tea catechins in full-fat cheese following gastrointestinal simulated digestion. J Food Compos Anal [Internet]. 2016;48:13–24. Available from: http://dx.doi.org/10.1016/j.jfca.2016.02.004
Moskaug JO, Carlsen H, Myhrstad MCW, Blomhoff R. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr. 2005;81(1 Suppl):277–83.
Roy P, George J, Srivastava S, Tyagi S, Shukla Y. Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors. Invest New Drugs. 2011;29(2):225–31.
Camihort G, Dumm CG, Luna G, Ferese C, Jurado S, Moreno G, et al. Relationship between pituitary and adipose tissue after hypothalamic denervation in the female rat: A morphometric immunohistochemical study. Cells Tissues Organs. 2005;179(4):192–201.
Winuthayanon W, Bernhardt ML, Padilla-Banks E, Myers PH, Edin ML, Lih FB, et al. Oviductal estrogen receptor α signaling prevents protease-mediated embryo death. Elife. 2015;4:1–28.
Wahyuni E, Situmorang C, Yueniwati Y, Barlianto W, Dwijayasa P. Combination of vitamin C and E modulated monosodium glutamate-induced endometrial toxicily in female Wistar rats. Asian Pacific J Reprod. 2014;3(2):106–9.
Mahmood B, Mokhtar M, Esfandiar S. The impact of green tea (camellia sinensis) on the amount of gonadotropin hormones (LH, FSH) in immature female rats poisoned with cadmium chloride. Biomed Pharmacol J. 2015;8(1):261–8.
Hoseini shahr khafri M alsadat, Hemayatkhah jahromi V, Samani Jahromi E. Protective effect of green tea extract on ovary tissue function in rats treated by Malathion insecticide. Pars Jahrom Univ Med Sci. 2015;13(3):52–64.
Cabrera C, Artacho R, Gime R. Beneficial Effects of Green Tea — A Review Beneficial Effects of Green Tea — A Review. 2006;(June 2018).
DOI: https://doi.org/10.35971/jjhsr.v4i3.14873
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