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Analisis Kadar Metabolit Sekunder, Histokimia, dan Aktivitas Antioksidan Akar Acalypha indica L.
Abstract
Acalypha indica L. is a species of wild plant that has secondary metabolites are used as medicine, but the usage is not optimized yet. The objective of of this study was to determine secondary metabolites content, location and distribution of secondary metabolites, and also the capacity for antioxidant. Secondary metabolites content was determined included phenol, flavonoids, tannin of chloroform and methanol extracts from Acalypha indica L. root, using spectrophotometre. Histochemical test was done by making fresh sliced preparation which was reacted with reagent. Antioxidant activity was determined by using DPPH method. The result of this study showed that secondary metabolites content of chloroform extract, were phenol 9,89 ± 0,77 %GAE, flavonoids 5,87 ± 1,40 %QE, and tannin 3,33 ± 1,21 %GAE, while methanol extract were phenol 45,11 ± 4,86 %GAE, flavonoids 19,87 ± 0,61 %QE, and tannin 6,76 ± 0,31 %GAE. Histochemical test showed that phenol, flavonoids, tannin, and alkaloids were found in Acalypha indica L. root. Antioxidant activity showed the IC50 value was 161,81 ± 7,88 μg/mL for chloroform extract and 92,81 ± 4,33 μg/mL for methanol extract.
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Mursyidi, A. 1990. Analisis Metabolit Sekunder: Alkaloid. Yogyakarta: Proyek Pengembangan Pusat Fasilitas Bersama Antar Universitas (Bank Dunia XVII)-PAU Bioteknologi Universitas Gadjah Mada.
Dewick, P.M. (2009). Medicinal Natural Products: A Biosynthetic Approach, Third Edition. United Kingdom: John Wiley and Sons Ltd.
Wijayakusuma, H., Wirian, A.S., Yaputra, T., Dalimartha,S., dan Wibowo, B. (1992). Tanaman Berkhasiat Obat di Indonesia. Jakarta: Pustaka Kartini
Hutapea, J.R. et al. (1993). Inventaris Tanaman Obat Indonesia (II). Jakarta: Badan Penelitian dan Pengembangan Kesehatan-DepKes RI.
Handa, S.S., Rakesh, D.D. dan Vasisht, K. (2006). Compendium of Medicinal and Aromatic Plants ASIA. Italia: United Nations Industrial Development Organization and the International Centre for Science and High Technology.
Joy, P.P., Thomas, J., Mathew, S. Dan Skaria, B.P. (1998). Medicinal Plants. India: Kerala Agricultural University.
Mullick, A., Mandal, S., Bhattacharjee, R. dan Banerjee, A. (2013). In-Vitro Assay of Antioxidant and Antibacterial Activity of Leaf Extract and Leaf Derived Callus Extract of Acalypha indica L. International Journal of Pharmacy and Biological Sciences, 3(1): 504-510.
Woraratphoka, J., Intarapichet, K., & Indrapichate, K. (2012). Antioxidant Activity and Cytotoxicity of Six Selected, Regional, Thai Vegetables. American-Eurasian Journal of Toxicological Sciences 4 (2): 108-117.
Mythili, K., Reddy, C.U., Chamundeeswari, D., dan Manna, P.K. (2014). Determination of Total Phenol, Alkaloid, Flavonoid and Tannin in Different Extracts of Calanthe Triplicata. Journal of Pharmacognsoy and Phytochemistry, 2(2): April – June
Berlyn, G.P. dan Miksche, J.P. (1976). Botanical Microtechnique and Cytochemistry. Lowa: The Lowa State University Press.
Stahl, E. (1985). Analisis Obat Secara Kromatografi dan Mikroskopi. Bandung: ITB.
Nkumah, O.C., Esther, A.E., , Adimonyemma, R.N., Cletus, N.O., dan Iroka, C.F. (2016). Preliminary Phytochemical Screening on the Leave, Stem and Root of Acalypha Indica, The Pharmaceutical and Chemical Journal 3(3):8-11
Markham, K.R. (1988). Cara Mengidentifikasi Flavonoid. Terjemahan Kosasih Padmawinata. Bandung: Penerbit ITB
Upton, R., Graff, A., Jolliffe, G., Länger, R., Williamson, E. & Swisher, D. (2011). Botanical Pharmacognosy-Microscopic Characterization of Botanical Medicines. United State: CRC Press Taylor & Francis Group.
Evans, W.C. & Trease. (2009). Pharmacognosy, 15th edition. Edinburgh, London, New York, Philadelphia, St.Louis, Sydney, Toronto: Saunders Elsevier.
Antolovich, M., Prenzler, P.D., Patsalides, E., McDonald, S., dan Robards, K. (2002). Methods for Testing Antioxidant Activity. Analyst, 127: 183–198
Tirzitis, G. dan Bartosz, G. (2010). Determination of Antiradical and Antioxidant Activity: Basic Principles and New Insights. Acta Biochimica Polonica, 57(1): 139–142.
Molyneux, P. (2004). The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol., 26(2): Maret-April
Fidrianny, I., Nurfitri, H., Sukrasno. (2015). In Vitro Antioxidant Activities, Phenolic, Flavonoid, and Carotenoid Content From Different Polarity Extracts of Five Citrus Peels Using DPPH and Cuprac Method, Journal of Chemical and Pharmaceutical Research 7(4): 1525-1531
Shanmugapriya, R., Ramanathan, T. dan Thirunavukkarasu, P. (2011). Evaluation of Antioxidant Potential and Antibacterial Activity of Acalypha indica Linn. Using in vitro Model. Asian Journal of Biomedical and Pharmaceutical Sciences, 1(1): 18-22.
Balakhrisnan, N., Panda A.B., Raj, N.R., Shrivastava, a., dan Prathani, R. (2009). The Evaluation of Nitric Oxide Scavenging Activity of Acalypha Indica Linn Root. Asian Journal of Research in Chemistry, 2(2): April-June.
DOI: https://doi.org/10.37311/jsscr.v3i2.11376
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