Uji Aktivitas Antibakteri Mikroba Endofit Dari Buah Merah (Pandanus conoideus Lam.) terhadap Bakteri Patogen

Abstract

Endophyte microbes can produce bioactive compounds that can be used as medicinal raw materials. Red fruit is one of the typical fruits that grow in the Papua area that has a potential bioactive compound content but has not been widely studied. So the study aimed to isolate endophyte microbes from red fruits (Pandanus conoideus Lam.) and determine activity against pathogenic microbes. The study began with the isolation of endophytic microbes from red fruit plants (Pandanus conoideus Lam.) using medium Nutrient Broth and Potato Dextrose Broth to obtain endophyte bacteria and fungi, once isolated and purified, pure microbes are inoculated in a medium to tilt. To maximize secondary metabolites, each microbe is fermented for 14 days. Supernatan fermentation results are tested antibacterial activity against test bacteria by the method of diffusion agar.  The test microbes used are Eschericia coli, Staphylococcus aureus, Salmonella thyposa, Streptococcus mutans, Candida albicans.  Positive endophyte microbes inhibit fermented pathogenic bacteria to maximize the production of their secondary metabolitesMikroba endophyte which has the ability to inhibit pathogenic bacteria characterized macroscopically and microscopically. From the results of the study, 4 endophyte microbes were produced, 2 in the form of molds, and 2 microbes from bacteria. From the results of the study, bakery N2, can inhibit the bacteria E.coli, S. mutans, S, aureus, S. thyposa. Gram staining, indicating N2 is gram negative bacteria. So it can be concluded that the N2 endophyte bacteria of red fruit (Pandanus conoideus Lam.), have bioactive compounds that can inhibit pathogenic microbes.