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Evaluation of Maja Leaf Simplisia (Aegle marmelos L.) for Herbal Standardization: Physicochemical and Microbial Testing
Abstract
Standardization aims to determine the quality of maja leaf simplicia (Aegle marmelos L.) on non-specific parameters, which include drying shrinkage test, moisture content test, ash content test, specific gravity test, total plate count test, yeast mold count test, and heavy metal contamination test. This study uses descriptive qualitative and quantitative analytical methods. The sample in this study was taken from the village of Kedamean, Gresik Regency. The standardization results on non-specific parameters showed that the drying shrinkage was 0.423%, the moisture content was 0.77%, and the ash content was 6.62%. The total plate number test results were 0.6 × 107 colonies/mL, while the total yeast contamination number was 326.6 colonies/mL. Then, for testing for heavy metal contamination, Pb did not find contamination, and Cd obtained results of 4.60 mg/kg, Cu 268.21 mg/kg, and Zn 10.89 mg/kg. Based on the research conducted, maja leaf simplicia powder on non-specific parameters, namely drying shrinkage, water content, total ash content, specific gravity, total plate number, and heavy metal contamination Pb, Cd, and Zn has fulfilled the requirements and conditions indicating that the simplicia has good quality. The results suggest that the maja leaf simplisia met most non-specific parameter standards, except for yeast and copper levels, which exceeded the acceptable limits.
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Bhar, K., Mondal, S., & Suresh, P. (2019). An eye-catching review of aegle marmelos L. (golden apple). Pharmacognosy Journal,11 (2), 207-224. https://doi.org/10.5530/pj.2019.11.34
Balakumar, S., Rajan, S., Thirunalasundari, T., & Jeeva, S. (2011). Antifungal activity of Aegle marmelos (L.) Correa (Rutaceae) leaf extract on dermatophytes. Asian Pacific Journal of Tropical Biomedicine,1 (4), 309-312. https://doi.org/10.1016/S2221-1691(11)60049-X
Indonesia, D. K. (2017). Farmakope Herbal Indonesia. Jakarta: Kementrian Kesehatan Republik Indonesia.
Badan Pengawas Obat dan Makanan Republik Indonesia. (2019). Peraturan Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 32 Tahun 2019 tentang Persyaratan Keamanan dan Mutu Suplemen Kesehatan. Jakarta: BPOM RI.
] Balakumar, S., Rajan, S., Thirunalasundari, T., & Jeeva, S. (2011). Antifungal activity of Aegle marmelos (L.) Correa (Rutaceae) leaf extract on dermatophytes. Asian Pacific Journal of Tropical Biomedicine,1 (4), 309-312. https://doi.org/10.1016/S2221-1691(11)60049-X
] Ningsih, A. W., Azizah, M. N., & Sinaga, B. (2022). Standardisation of moringa leaf (Moringa oleifera L.) simplisia from luwung village sidoarjo using food dehydrator drying. Journal of Pharmaceutical & Herbal Research, 5(1), 76-85.
] Indonesia, B. P. (2006). Monografi Ekstrak Tumbuhan Obat Indonesia. Jakarta.
] Ngibad, et al. (2024). Standardization of simplicia and extracts of Arabic bidara (Ziziphus spina-christi (L.) Desf.) and tree saga (Adenanthera pavonina L.) leaves. Journal of Chemical Health Risks, Volume 14, https://jchr.org/index.php/JCHR/article/view/3748
] Badan Pengawas Obat dan Makanan Republik Indonesia. (2006). Pedoman umum pengawasan obat tradisional. Jakarta: BPOM RI.
] Zulharmita, Zulfaretna, M., & Misfadhila, S. (2017). Analysis of heavy metal contamination in herbal medicine preparations at Siti Rahmah Islamic Hospital Padang by atomic absorption spectrophotometry. Higea Pharmacy Journal,9 (2), 159-164. http://www.jurnalfarmasihigea.org/index.php/higea/article/view/171
Andasari, S., Hana Mustofa, C., & Oktavia Arabela, E. (2021). Standardisation of Specific and Non-Specific Parameters of Ethyl Acetate Extract of Beluntas Leaf (Pluchea indica L.). Journal of Pharmaceutical Sciences, 12(1), 47–53. https://doi.org/10.61902/cerata.v12i1.252
Hidayati, D. N., Sumiarsih, C., & Mahmudah, U. (2005). Non-specific standardisation of ethanol extract of Berenuk leaf and stem bark. Scientific Journal of Cendekia Eksakta, 19–23.
Dharma, M. A., Nocianitri, K. A., Luh, N., Yusasrini, A., & Ilmu, J. (2020). Journal of Food Science and Technology Effect of Simplisia Drying Method To The Antioxidant Capacity Of Wedang Uwuh Student of Food Science and Technology Study Programme, Faculty of Tech. 9(1), 88–95.
Afriliah, N., Taurina, W., & Andrie, M. (2022). Characterisation of Simplisia of Kelulut Honey (Heterotrigona itama) as Raw Material for Wound Healing Drug Preparation. Pharmacy and Pharmacology Magazine,26 (3), 104-110. https://doi.org/10.20956/mff.v26i3.20969
Utami, Y. P., Umar, A. H., Syahruni, R., & Kadullah, I. (2017). Standardisation of Simplisia and Ethanol Extract of Leilem Leaf (Clerodendrum. 2(1), 32–39.
Riza Linda, G. W. R. (2019). Total Microbial Plate Numbers in Tea Drinks in Pontianak City. Protobiont Journal,8 (2), 69-73. https://doi.org/10.26418/protobiont.v8i2.33968
Sinaga, B. (2021). Effect of Drying Method on the Quality of Red Guava Leaf Simplisia (Psidium guajava L.). Jurnal Jamu Kusuma,1 (2), 67-75. https://doi.org/10.37341/jurnaljamukusuma.v1i2.12
] Puskesmas, D. I., & Ahmad, R. (2022). Standardisation of the Manufacturing Process of Dasawisma Matahari Herbal Powder Used as an Alternative Medicine at Puskesmas Rasimah Ahmad Bukittinggi. Endurance Journal,7 (1), 128-137. https://doi.org/10.22216/jen.v7i1.789
] Saweng, C. F. I. J., Sudimartini, L. M., & Suartha, I. N. (2020). Microbial Contamination Test on Neem Leaves (Azadiractha Indica A. Juss) as a Standardisation of Herbal Medicinal Ingredients. Indonesia Medicus Veterinus,9 (2), 270-280. https://doi.org/10.19087/imv.2020.9.2.270
Putri, A., Sudimartini, L. M., & Dharmayudha, A. A. G. O. (2020). Standardisation of Microbial Contamination of Soursop Leaves (Annona muricata L.) as Raw Material for Traditional Medicinal Preparations. Indonesia Medicus Veterinus,9 (3), 305-313. https://doi.org/10.19087/imv.2020.9.3.305
Sari, R. I., Dewi, S., & Wilson, W. (2020). Total Microbes of Herbs Packaging and Without Packaging Product Banjarmasin. Journal of Health Analyst Media,11 (1), 1-10. http://journal.poltekkes-mks.ac.id/ojs2/index.php/mediaanalis/article/view/1298
Andriani, D., & Kusuma, E. W. (2019). Analysis of Cu and Zn Metals in Red Betel Leaf Extract by Atomic Absorption Spectrophotometry. Research Fair Unisri,3 (Vol 3, No 1 (2019)), 1-3.
Andriani, D., Puspitasari, D., Pratimasari, D., Farmasi, P. S., Tinggi, S., & Nasional, I. K. (2023). Heavy Metal Content of Pb, Cd, Hg, As in Telang Flowers (Clitoria ternatea L.) from Sleman Regency, Yogyakarta Special Region. Medihealth: Journal of Health and Science,3 (NO 01), 58-65. http://jurnalmbp.org/index.php/Medihealth/article/view/56
Syafitri, M. H., Sari, A. M., Ermawati, N. Y., & Wati, M. R. (2022). Characteristic Test of Simplisia and Ethanol Extract of Javanese Chilli Fruit that Grows in Jember Area. Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS),4 (01), 27. https://doi.org/10.30587/herclips.v4i01.4544
Khairuddin, Yamin, M., & Syukur, A. (2019). Penyuluhan Tentang Sumber-Sumber Kontaminan Logam Berat Pada Siswa SMAN 1 Belo . Jurnal Pendidikan dan Pengabdian Masyarakat, 2(1), pp. 64-71.
DOI: https://doi.org/10.37311/jsscr.v7i2.31220
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