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Formulation and In Vivo Evaluation of Hulu’u Fish (Giuris margariticus) Albumin Spray Gel for Diabetic Wounds
Abstract
This study investigates the potential of Hulu'u fish (Giuris margariticus) albumin as a wound-healing agent for diabetic wounds through the development of HulDerma Spray, a spray gel formulation derived from Hulu'u fish albumin. The gel was prepared with varying concentrations of fish albumin (10%, 15%, and 30%), along with excipients such as propylene glycol, hydroxypropyl methylcellulose (HPMC), and DMDM hydantoin. Diabetic wounds were induced in male Wistar rats using an intraperitoneal injection of streptozotocin (STZ), and the healing process was evaluated over a 7-day period. The study focused on assessing the physical stability of the formulations, including viscosity, pH, spreadability, and spray coverage, along with their effectiveness in promoting wound healing. The physical stability results showed minimal changes in both viscosity (±1.77 to ±2.07) and pH (±0.17 to ±0.36) throughout the freeze-thaw cycles, indicating that the formulations maintained their consistency and suitability for topical application. Wound healing efficacy was evaluated by measuring the wound diameter reduction. The 30% albumin formulation (F3) exhibited the most significant reduction in wound diameter, with a 50% decrease, from 20 mm to 9.4 mm by Day 7. The Negative Control group, treated with the base formulation without albumin, showed a slower healing rate, with a wound diameter reduction from 20 mm to 12.7 mm. This confirms that the albumin-based formulations contribute to accelerated wound healing. The findings of this study suggest that HulDerma Spray gel, particularly the 30% albumin formulation, has a significant potential for enhancing wound healing in diabetic rats. This supports the use of Hulu'u fish albumin as a promising therapeutic agent in diabetic wound care, offering an innovative solution for improving wound recovery while utilizing local, sustainable resources. The results confirm the efficacy of the formulations in accelerating wound closure, making HulDerma Spray a viable option for diabetic wound treatment.
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J. Holl, C. Kowalewski, Z. Zimek, P. Fiedor, A. Kaminski, T. Oldak, and P. Fiedor, “Chronic diabetic wounds and their treatment with skin substitutes,” Cells, vol. 10, no. 3, 655, 2021. [Online]. Available: https://doi.org/10.3390/cells10030655
J. L. Burgess, W. A. Wyant, B. A. Abujamra, R. S. Kirsner, and I. Jozic, “Diabetic wound-healing science,” Medicina, vol. 57, no. 10, 1072, 2021. [Online]. Available: https://doi.org/10.3390/medicina57101072
Y. Xiao, Y. Chen, and X. Hu, “Innovations in hydrogel therapies for diabetic wound healing: Bridging the gap between pathophysiology and treatment,” Int. J. Mol. Sci., vol. 26, no. 2, 912, 2025. [Online]. Available: https://doi.org/10.3390/ijms26020912
G. Suarato, R. Bertorelli, and A. Athanassiou, “Borrowing from nature: Biopolymers and biocomposites as smart wound care materials,” Front. Bioeng. Biotechnol., vol. 6, 137, 2018. [Online]. Available: https://doi.org/10.3389/fbioe.2018.00137
M. Ansari and A. Darvishi, “A review of the current state of natural biomaterials in wound-healing applications,” Front. Bioeng. Biotechnol., vol. 12, 1309541, 2024. [Online]. Available: https://doi.org/10.3389/fbioe.2024.1309541
N. I. Fadilah, S. J. Phang, N. Kamaruzaman, and A. Salleh, “Antioxidant biomaterials in cutaneous wound healing and tissue regeneration: A critical review,” Antioxidants, vol. 12, no. 4, 787, 2023. [Online]. Available: https://doi.org/10.3390/antiox12040787
L. Wang, Y. Qu, W. Li, K. Wang, and S. Qin, “Effects and metabolism of fish collagen sponge in repairing acute wounds of rat skin,” Front. Bioeng. Biotechnol., vol. 11, 1087139, 2023. [Online]. Available: https://doi.org/10.3389/fbioe.2023.1087139
A. Pesterau, A. Popescu, R. Sîrbu, E. Cadar, F. Busuricu, M. Radu, and C. G. Mihăescu, “Marine jellyfish collagen and other bioactive natural compounds from the sea with significant potential for wound healing and repair,” Mar. Drugs, vol. 23, no. 6, 252, 2025. [Online]. Available: https://doi.org/10.3390/md23060252
A. Iosageanu, A. Oancea, D. Ilie, E. Daniela, and O. Crăciunescu, “The effect of fish bone bioactive peptides on the wound-healing process: An in vitro study on keratinocytes,” Romanian Biotechnol. Lett., vol. 26, no. 3, pp. 2692–2699, 2021. [Online]. Available: https://doi.org/10.25083/rbl/26.3/2692.2699
A. Herman and A. P. Herman, “Herbal products and their active constituents for diabetic wound healing—Preclinical and clinical studies: A systematic review,” Pharmaceutics, vol. 15, no. 1, 281, 2023. [Online]. Available: https://doi.org/10.3390/pharmaceutics15010281
J. Reveny, S. E. K. A. Nugraha, and A. Kamilia, “Formulation of anti-diabetic ulcer gel enriched with Aloe vera extract and fish collagen: An in vivo study,” Int. J. App. Pharm., vol. 17, no. 4, pp. 202–210, 2025. [Online]. Available: https://doi.org/10.22159/ijap.2025v17i4.51149
N. Hou and B. Chen, “Preparation of nanoemulsions with low-molecular-weight collagen peptides from sturgeon fish skin and evaluation of anti-diabetic and wound-healing effects in mice,” Pharmaceutics, vol. 15, no. 9, 2304, 2023. [Online]. Available: https://doi.org/10.3390/pharmaceutics15092304
L. Alparslan, “Smart thermoresponsive sol–gel formulation of polyhexanide for rapid and painless burn and wound management,” Polymers, vol. 17, no. 15, 2079, 2025. [Online]. Available: https://doi.org/10.3390/polym17152079
R. Smith, N. Brogden, and J. Fiegel, “Sprayable ciprofloxacin-loaded poloxamer hydrogels for wound infection treatment,” J. Drug Deliv. Sci. Technol., vol. 89, 105000, 2024. [Online]. Available: https://doi.org/10.1016/j.jddst.2023.105000
A. Rungrod, A. Makarasen, S. Patnin, and S. Techasakul, “Design and development of a sprayable hydrogel based on thermo/pH dual-responsive polymer incorporating Azadirachta indica (Neem) extract for wound dressing applications,” Polymers, vol. 17, no. 15, 2157, 2025. [Online]. Available: https://doi.org/10.3390/polym17152157
M. Konwarh, “Embracing sustainable processes in the pharmaceutical industry with green chemistry and engineering,” Sustainable Processes, vol. 1, 133442, 2025. [Online]. Available: https://doi.org/10.69709/SusProc.2025.133442
P. García-Pérez, E. Lozano-Milo, M. Landin, and P. P. Gallego, “From ethnomedicine to plant biotechnology and machine learning: The valorization of the medicinal plant Bryophyllum sp.,” Pharmaceuticals, vol. 13, no. 12, art. no. 444, 2020. [Online]. Available: https://doi.org/10.3390/ph13120444
S. T. Gonapinuwala, S. K. Jones, and M. D. Bronlund, “Collagen with native triple-helical structure from representative cold-water and warm-water fish skins using a modified protocol,” Food Bioprocess Technol., 2025. [Online]. Available: https://doi.org/10.1007/s11947-025-03912-8
A. Rekowski, G. Langenkämper, K. A. Scherf, C. Zörb, and M. A. Wimmer, “Determination of soluble wheat protein fractions using the Bradford assay,” Cereal Chem., vol. 98, no. 5, pp. 1059–1065, 2021. [Online]. Available: https://doi.org/10.1002/cche.10447
E. A. Ozon, A. M. Burloiu, A. M. Musuc, G. Manda, V. Anuţa, C. E. Dinu-Pîrvu, and C. V. Cristea, “Cellulose-derived gels for topical delivery: HPMC as a functional matrix for porphyrinic photosensitizers,” Gels, vol. 11, no. 10, 824, 2025. [Online]. Available: https://doi.org/10.3390/gels11100824
D. Luzio, S. K. De, and P. Ferreira, “Preparation of gel-forming polymer-based sprays for first-aid care of skin injuries,” Polymers, vol. 16, no. 4, 743, 2024. [Online]. Available: https://doi.org/10.3390/polym16040743
M. Lukić, I. Pantelić, and S. D. Savić, “Towards optimal pH of the skin and topical formulations: From the current state of the art to tailored products,” Cosmetics, vol. 8, no. 3, 69, 2021. [Online]. Available: https://doi.org/10.3390/cosmetics8030069
M. Sieben, R. Hanke, and J. Büchs, “Contact-free determination of viscosity in multiple parallel samples,” Sci. Rep., vol. 9, 10408, 2019. [Online]. Available: https://doi.org/10.1038/s41598-019-44859-z
A. Potanin and G. Marron, “Rheological characterization of yield-stress fluids with Brookfield viscometer,” Appl. Rheol., vol. 31, no. 1, 13113, 2021. [Online]. Available: https://doi.org/10.1515/arh-2021-0001
Y. Rizikiyan, F. F. Nur, I. Suharyani, and R. Amelia, “Formulation and antioxidant activity test of peel off gel mask kombucha bunga telang using the DPPH method,” Pharmacoscript, vol. 8, no. 2, pp. 544–555, 2025. [Online]. Available: https://doi.org/10.36423/pharmacoscript.v8i2.2079
Y. He, J. Wu, H. Fu, Z. Sun, H. Fang, and X. Sun, “Quantitative analysis of droplet size distribution in plant protection spray based on machine-learning method,” Water, vol. 14, no. 2, 175, 2022. [Online]. Available: https://doi.org/10.3390/w14020175
D. Koo, C. A. Henderson, and S. D. Askew, “Agricultural spray drone deposition, part 1: Methods for high-throughput spray pattern analysis,” Weed Sci., vol. 72, no. 1, pp. 31–44, 2024. [Online]. Available: https://doi.org/10.1017/wsc.2024.66
I. J. Joye, “Peak fitting applied to Fourier-transform infrared and Raman spectroscopic analysis of proteins,” Appl. Sci., vol. 10, no. 21, 7364, 2020. [Online]. Available: https://doi.org/10.3390/app10217364
P. M. Donaldson, “Photon echoes and two-dimensional spectra of the amide I band of proteins measured by femtosecond IR–Raman spectroscopy,” Chem. Sci., vol. 11, no. 33, pp. 8862–8874, 2020. [Online]. Available: https://doi.org/10.1039/D0SC02978E
J. Seelig and A. Seelig, “Protein stability: Analysis of heat and cold denaturation without and with unfolding models,” J. Phys. Chem. B, vol. 127, no. 30, pp. 6797–6811, 2023. [Online]. Available: https://doi.org/10.1021/acs.jpcb.3c00882
I. V. Agafonkina, I. A. Korolev, and T. A. Sarantsev, “The study of thermal denaturation of beef, pork, chicken and turkey muscle proteins using differential scanning calorimetry,” Foods and Raw Materials, vol. 7, no. 1, pp. 19–23, 2019. [Online]. Available: https://doi.org/10.21323/2308-4057-2019-7-1-19-23
K. Cheng, Z. Lin, Y. Cheng, H. Chiu, and N. Yeh, “Wound healing in streptozotocin-induced diabetic rats using atmospheric-pressure argon plasma jet,” Sci. Rep., vol. 8, 12258, 2018. [Online]. Available: https://doi.org/10.1038/s41598-018-30597-1
A. Ghasemi, “Streptozotocin as a tool for induction of rat models of diabetes: A practical guide,” J. Basic Clin. Pathophysiol., vol. 11, no. 2, pp. 7–22, 2023. [Online]. Available: https://doi.org/10.32598/jbcp.11.2.7
P . Khoshnoudi, “Exploring effect of M2 macrophages on experimental full-thickness wound healing in streptozotocin-induced diabetic rats,” Int. Wound J., vol. 21, no. 1, pp. 13–20, 2024. [Online]. Available: https://doi.org/10.1111/iwj.12496
H. Setiawan, M. Intanon, and P. Chummitri, “Protein concentrate: Excellent recovery of fish-based albumin source and its possible application for sperm capacitation,” Asian Fish. Sci., vol. 37, no. 2, pp. 125–137, 2024. [Online]. Available: https://doi.org/10.33997/j.afs.2024.37.2.005
S. W. Sidauruk and T. Hidayat, “Isolation and characterization of snakehead fish meal extract with fresh, boiled, and steamed treatments and its potential for health drinks and immunomodulators,” Pharmacogn. J., vol. 14, no. 1, pp. 532–536, 2022. [Online]. Available: https://doi.org/10.5530/pj.2022.14.131
K. Dirga, M. Buana, L. N. Wiwin, and A. I. Suryani, “Effect of hydroxypropyl methylcellulose concentration variations as a gelling agent on the physical quality of hydrogel formulations containing ethanol extract of Impatiens balsamina L. herb,” Int. J. Med. Eng. Dev., vol. 10, no. 4, pp. 267–277, 2025. [Online]. Available: https://doi.org/10.26911/theijmed.2025.10.4.872
D. Puspitasari and E. Suprayitno, “The effect of giving fish cork albumin gel (Channa striata) to the mice wound closure,” Int. J. Sci. Res. Publ., vol. 10, no. 7, pp. 571–578, 2020. [Online]. Available: https://doi.org/10.29322/IJSRP.10.07.2020.p10360
T. Triwiyono, Y. Husodo, and E. Suprayitno, “Effect of sunflower seed oil (Helianthus annuus L.) addition as an omega-6 source in snakehead fish (Channa striata) albumin gel on wound closure,” Int. J. Sci. Res. Publ., vol. 11, no. 7, pp. 330–336, 2021. [Online]. Available: https://doi.org/10.29322/IJSRP.11.07.2021.p11541
T. Thangarajoo, Y.-K. Hsin, M. Pandey, H. Choudhury, L.-W. Meng, and B. Gorain, “A stimuli-responsive in situ spray hydrogel co-loaded with naringenin and gentamicin for chronic wounds,” Chem. Pap., vol. 77, no. 12, pp. 1–19, 2023. [Online]. Available: https://doi.org/10.1515/chem-2022-0357
J. Jeong, K. Kim, J. Kim, Y. Kim, and S. Jung, “New polyvinyl alcohol/succinoglycan-based hydrogels for pH-responsive drug delivery,” Polymers, vol. 15, no. 14, 3009, 2023. [Online]. Available: https://doi.org/10.3390/polym15143009
S. Wongtechanon, C. Noosak, P. Jantorn, P. Watcharanurak, P. Swangphon, W. Wanna, et al., “Innovative sericin-based film-forming gel for wound healing: Development and performance evaluation,” Polymers, vol. 17, no. 9, 1246, 2025. [Online]. Available: https://doi.org/10.3390/polym17091246
M. S. Naji, T. A. Beig, B. J. E. Moros, M. C. Sánchez, and J. M. Franco, “Improving the structure and properties of whey-protein emulsion gel using soluble interactions with xanthan and basil-seed gum,” Food Sci. Nutr., vol. 11, no. 10, pp. 6907–6919, 2023. [Online]. Available: https://doi.org/10.1002/fsn3.3598
Y. Elsaboni, J. A. Hunt, J. Stanley, C. Moffatt, and Y. Wei, “Development of a textile-based protein sensor for monitoring the healing progress of a wound,” Sci. Rep., vol. 12, 9801, 2022. [Online]. Available: https://doi.org/10.1038/s41598-022-11982-3
W. Wei, W. Hu, X. Zhang, F. Zhang, S. Sun, and Y. Liu, “Analysis of protein-structure changes and quality regulation of surimi during gelation based on infrared spectroscopy and microscopic imaging,” Sci. Rep., vol. 8, 12426, 2018. [Online]. Available: https://doi.org/10.1038/s41598-018-23645-3
E. Fonseca, S. Fábia, and H. Sander, “Synthesis and characterization of poly(vinyl alcohol) hydrogels and hybrids for rMPB70 protein adsorption,” Mater. Res., vol. 9, no. 2, pp. 185–191, 2006. [Online]. Available: https://doi.org/10.1590/S1516-14392006000200014
N. A. Taslim, C. P. Marsella, A. Bukhari, M. H. Cangara, A. M. Aman, and A. Aminuddin, “The effect of snakehead fish extract on the acute wound-healing process in hyperglycemic rats,” Front. Vet. Sci., vol. 11, 1456789, 2024. [Online]. Available: https://doi.org/10.3389/fvets.2024.1456789
M. Mieczkowski, B. Mrozikiewicz-Rakowska, M. Kowara, and M. Kleibert, “The problem of wound healing in diabetes—From molecular pathways to the design of an animal model,” Int. J. Mol. Sci., vol. 23, no. 14, 7930, 2022. [Online]. Available: https://doi.org/10.3390/ijms23147930
B. Yuliana, N. Djide, and Y. Y. Djabir, “Wound-healing effect of snakehead fish (Channa striata) mucus-containing transdermal patch,” J. App. Pharm. Sci., vol. 12, no. 7, pp. 171–183, 2022. [Online]. Available: https://doi.org/10.7324/JAPS.2022.120717
DOI: https://doi.org/10.37311/jsscr.v8i1.34957
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