Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Daun Mengkudu (Morinda citrifolia L.) Terhadap Staphylococcus aureus

Fajrin Noviyanto, Siti Nuriyah, Hadi Susilo

Abstract


The bacteria Staphylococcus aureus are round bacteria that are shaped like a cocci, and are Gram-positive. These bacteria can be found on the skin, genitals, nasal cavity, mouth and around the anus. One of the pharmaceutical products that can maintain skin health and protect the skin is soap. The leaves of noni (M. citrifolia L.) contain various compounds such as flavonoids, tannins, saponins, alkaloids, and triterpenoids which are capable of being antibacterial. This study aims to make a liquid soap formulation from M. citrifolia L. leaf extract and its activity as an antibacterial to S aureus. The leaves of M. citrifolia L. were extracted using 96% ethanol solvent by maceration method until a thick extract was obtained. Soap formulations are made with a concentration of 20% 40% and 60%. Evaluation of soap quality includes organoleptic test, high foam test and pH test. The method of antibacterial testing is carried out in vitro by means of well diffusion. The results on the quality test of the liquid soap formulation showed that the pH and height of the foam had good results and had met the SNI 1996 Standards. From the results showed that formulation III with an extract content of 60% had a large antimicrobial activity with a value of 16.83 mm which was categorized as strong. In positive control (Dettol) 60% antimicrobial activity showed a value of 14.83 mm. The results of statistical tests were carried out by One-Way ANOVA analysis followed by LSD analysis Post hoc, liquid soap extract of M. citrifolia L. had greater antimicrobial potential than the positive control (Dettol).


Keywords


Staphylococcus aureus, morinda citrifolia L., Antibacterial, Liquid Soap.

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References


. Triana, D., 2017. Frekuensi β-Lactamase Hasil Staphylococcus aureus Secara Iodometri di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jurnal Gradien, 10 ( 2) : 992-995

. Tyaningsih, dkk., 2010. Buku Ajar Penyakit Infeksius I. Airlangga University Press: Surabaya.

. Nismawati., R., Sjahril, R., & Agus, R., 2018. Deteksi Methicillin Resistant Staphylococcus aureus (MRSA) Pada Pasien Rumah Sakit Universitas Hasanuddin Dengan Metode Kultur. Jurusan Biologi, UIN Alaudin Makasar, 15- 21. ISBN: 978-602-72245-3-7. doi: https://doi.org/10.24252/psb.v4i1.5932

. Kasenda, J. C., YamLean, P. V. Y., & Lolo, W. A., 2016. Formulasi dan Pengujian Aktivitas Antibakteri Sabun Cair Ekstrak Etanol Daun Ekor Kucing (Acalypha hispida Burm. F) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Ilmiah Farmasi 5 (3) : 40-47. doi: https://doi.org/10.35799/pha.5.2016.12936

. Dimpudus, S. A., Yamlean, P. V. Y., & Yudistira, A. 2017. Formulasi Sediaan Sabun Cair Antiseptik Ekstrak Etanol Bunga Pacar Air (Impatiens balsamina L.) dan Uji Efektivitasnya Terhadap Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Ilmiah Farmasi, 6 (3): 208-215. doi: https://doi.org/10.35799/pha.6.2017.16885.

. Afiff, F. E., & Amilah, S., 2017. Efektivitas Ekstrak Daun Mengkudu (Morinda citrifolia L.) dan Daun Sirih Merah (Piper Crocatum Ruiz & Pav) Terhadap Zona Hambat Pertumbuhan Staphylococcus aureus. Journal of Science, 10 (1): 12-16. ISSN: 1412 – 1840.

. Simatupang, O. C., Abidjulu, J., & Siagian, K. V., 2017. Uji daya hambat ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans secara in vitro. Jurnal e-GiGi (eG), 5 (1) : 1-6. doi:https://doi.org/10.35790/eg.5.1.2017.14701

. Erina, dkk. 2019. Uji Daya Hambat Ekstrak Etanol Daun Mengkudu (Morinda citrifolia L.) Terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kedokteran, Universitas Syiah Kuala. 3(3):161-169. doi: https://doi.org/10.21157/jim%20vet..v3i3.11377

. Lestari, I., & Hanum, G. R., 2019. Aktivitas Antibakteri Kombinasi Ekstrak Daun Mengkudu (Morinda citrifolia L.) dan Bawang Putih (Allium sativum) terhadap Escherichia coli dan Staphylococcus aureus. Journal of Medical Laboratory Science Technology, 2 (2) : 43-47. doi: 10.21070/medicra.v2i2.1475

. Herawati, A., & Amelia, T. R. N., 2018. Potensi Bahan Herbal Ekstrak Etanol Daun Mengkudu Asal Desa Wajak Lor, Tulungagung, Jawa Timur Terhadap Bakteri Penyebab Jerawat. Jurnal JuKe 2 (2) : 173-178.

. Misna, & Diana, K., 2016. Aktivitas Antibakteri Ekstrak Kulit Bawang Merah (Allium Cepa L.) Terhadap Bakteri Staphylococcus aureus. Jurnal. Journal of pharmacy 2 (2) : 138-144. doi: 10.22487/j24428744.2016.v2.i2.5990

. Handayani, F., Sundu, R., & Sari, R. M., 2017. Formulasi dan Uji Aktivitas Antibakteri Streptococcus mutans Dari Sediaan Mouthwash Ekstrak Daun Jambu Biji (Psidium guajava L.). Jurnal Sains dan Kesehatan 1 (8) : 422-433. doi: https://doi.org/10.25026/jsk.v1i8.62

. Ferdinan, A., & Sari, R., 2017. Pengujian Aktivitas Antibakteri Sabun Cair dari Ekstrak Kulit Daun Lidah Buaya. Pharm Sci Res, 4 (3): 11-120. doi: 10.7454/psr.v4i.3763

. Harbone, J. B., 1996. Metode Fitokimia Penuntun cara Modern Menganalisis Tumbuhan, diterjemahkan oleh Kosasih P. & Imam S. Edisi II, Hal 4-7: 69-76, ITB, Bandung.

. Wijaya, H., Novitasari., & Jubaidah, S., 2018. Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambai Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1) : 79-83.

. Naiu, A. S., & Yusuf, N., 2018. Nilai Sensoris Dan Viskositas Skin Cream Menggunakan Gelatin Tulang Tuna Sebagai Pengemulsi Dan Humektan. Jurnal PHPI, 21 (2): 199-207. doi: https://doi.org/10.17844/jphpi.v21i2.22838

. SNI 06-4085-1996, Standar Mutu Sabun Mandi Cair, Dewan Standarisasi Nasional : Jakarta.




DOI: https://doi.org/10.37311/jsscr.v2i2.7016

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