Terpenoid dari Fraksi n-Heksan dan Uji Aktivitas Antioksidan Daun Singkil (Premna serratifolia Linn)

Isnindar Isnindar, Sri Luliana, Muhammad Zahid

Abstract


Singkil (Premna serratifolia Linn.) is a plant that has a distinctive aroma and is edible. The monoterpene and sesquiterpene content found in the singkil plant has the potential to be an active substance with medicinal benefits. This research aims to isolate, identify and test the antioxidant activity of terpenoid compounds in the n-hexane fraction of singkil leaves using the GC-MS method. Singkil leaves were extracted by maceration with 96% ethanol solvent and then liquid-liquid fractionated using n-hexane and ethyl acetate solvents. Hexane obtained from singkil leaves was isolated via column chromatography using a gradient mobile phase of n-hexane and ethyl acetate and tested for antioxidant activity using the DPPH method. The isolates obtained were identified as 20 compounds, among these compounds were 12 compounds belonging to the terpenoid group, including 2-Nonanol, 2,4-heptadienal Terpenoid, 3- Ethyl-Santolina Triene, 2,4-Decadienal, Nonyl-Cetene, 2 ,4,6-Trimethyl-1-heptadecene, 3- Tetramethyl-2-Hexadecen-1-ol, 1-Docosene, 13-tetradecen-11-yn-1-ol, and Cyclohexane. The antioxidant test results showed that the n-Hexane fraction had a % inhibition value of 53.078%.

Keywords


Singkil leaves; Ethanol extract; n-hexane fraction; GC-MS

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References


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DOI: https://doi.org/10.37311/ijpe.v4i2.26860

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