Uji Aktifitas Antioksidan, flavonoid Total dan Formulasi Sediaan Krim Ekstrak Bangle (Zingiber cassumunar)

Munifatul Lailiyah, Sony Andika Saputra, Dyah Aryantini

Abstract


Bangle rhizome (Zingiber cassumunar) is a plant with essential oil, flavonoid, and curcumin content which is efficacious as a traditional medicine and has antioxidant activity. The purpose of this study was to determine the antioxidant activity and test the total flavonoid content contained in the rhizome extract (Zingiber cassumunar) using UV-Vis spectrophotometry. Then a cream preparation was made and tested for physical quality including, organoleptic, homogeneity, pH, adhesive power, and spreadability. The extraction method uses the maceration method of bangle rhizomes (Zingiber cassumunar) using an alcohol solvent until a thick extract is obtained, then a quantitative test is carried out for the content of total flavonoid compounds in the form of determining total flavonoid levels and free radical scavenging activity using the DPPH method. The results showed that the bangle rhizome extract (Zingiber cassumunar) had a yield value of 26.25%. The extract of bangle rhizome (Zingiber cassumunar) has a total flavonoid value of 86.7±0.4 mg QE/g extract, which means it contains high flavonoid compounds and has an IC50 value of 48.29±7.9 (µg/mL) with a powerful category. The physical quality test of the three formulations meets the criteria for physical quality test requirements. The total flavonoid compound content value in the 96% ethanol extract of bangle rhizome (Zingiber cassumunar) is 86.7 mg QE / g extract which means it contains high flavonoid compounds. The IC50 value of the 96% ethanol extract of bangle rhizome (Zingiber cassumunar) is 48.29µg/mL which means it has extreme free radical scavenging activity. Formulations, 1, 2, and 3 meet the physical quality test.

Keywords


Bangles; Antioxidant; Total flavonoids; Cream

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References


A. N. Pratama and H. Busman, “Potensi antioksidan kedelai (Glycine Max L) terhadap penangkapan radikal bebas,” J. Ilm. Kesehat. Sandi Husada, vol. 11, no. 1, pp. 497–504, 2020.

E. O. A. A. Jaya, A. Ahwan, and F. Qonitah, “Uji Aktivitas Antioksidan pada Ekstrak Etanol Kulit Batang Mahoni (Swietenia mahagoni (L.) JACQ) Daerah Sragen dan Karanganyar dengan Menggunakan Metode DPPH (1, 1-Difenil-2-Pikrihidazil),” Universitas Sahid Surakarta, 2023.

L. N. P. Anggi, “Uji Aktivitas Antioksidan Formulasi Sediaan Krim Wajah Ekstrak Etanol Daun Bayam Hijau (Amaranthus Hybidrus L.),” Universitas Mahasaraswati Denpasar, 2024.

S. N. Ashari, H. H. Pramesti, I. Fitriana, and S. Rohmani, “Potensi Senyawa Flavonoid dalam Tanaman sebagai Lotion Tabir Surya,” in Proceedings National Conference PKM Center, 2021, vol. 1, no. 1.

D. F. Alhabsyi, “Aktivitas antioksidan dan tabir surya pada ekstrak kulit buah pisang goroho (Musa acuminate L.),” Pharmacon, vol. 3, no. 2, 2014.

A. R. Abubakar and M. Haque, “Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes,” J. Pharm. Bioallied Sci., vol. 12, no. 1, pp. 1–10, 2020.

S. Zahrah Muhafidzah and R. A. Syarif, “AKTIVITAS ANTIOKSIDAN FRAKSI RIMPANG KENCUR (Kaempferia rhizoma) DENGAN MENGGUNAKAN METODE PEREDAMAN 1, 1 Diphenyl-2-picrylhydrazil (DPPH)”.

D. Susiloningrum and D. E. M. Sari, “OPTIMASI SUHU UAE (Ultrasonik Asssisted Extraction) TERHADAP NILAI SUN PROTECTION FACTOR (SPF) EKSTRAK RIMPANG BANGLE (Zingiber Purpureum Roxb) SEBAGAI KANDIDAT BAHAN AKTIF TABIR SURYA,” Cendekia J. Pharm., vol. 7, no. 1, pp. 58–66, 2023.

I. M. S. Adiputra et al., Metodologi penelitian kesehatan. Yayasan Kita Menulis, 2021.

N. Kemit, I. W. R. Widarta, and K. A. Nocianitri, “KANDUNGAN SENYAWA FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN ALPUKAT ( Persea Americana Mill ),” Fak. Teknol. Pertan. Univ. Udayana, pp. 130–141, 2010.

D. Aryantini, P. Astuti, N. Yuniarti, and S. Wahyuono, “Extraction and Isolation of Phytochemicals from Kaempferia rotunda Linn. (White Turmeric) for Pharmacological Application: A Review,” Trop. J. Nat. Prod. Res., vol. 6, no. 9, pp. 1359–1366, Sep. 2022, doi: 10.26538/TJNPR/V6I9.2.

A. Abubakar and M. Haque, “Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes,” J. Pharm. Bioallied Sci., vol. 12, no. 1, p. 1, Jan. 2020, doi: 10.4103/jpbs.JPBS_175_19.

T. W. Senduk, L. A. D. Y. Montolalu, and V. Dotulong, “The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba,” J. Perikan. Dan Kelaut. Trop., vol. 11, no. 1, pp. 9–15, 2020.

I. K. Widhiana Putra, G. . Ganda Putra, and L. P. Wrasiati, “Pengaruh Perbandingan Bahan dengan Pelarut dan Waktu Maserasi terhadap Ekstrak Kulit Biji Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan,” J. Rekayasa Dan Manaj. Agroindustri, vol. 8, no. 2, p. 167, 2020, doi: 10.24843/jrma.2020.v08.i02.p02.

G. A. Nayik, B. N. Dar, and V. Nanda, “Optimization of the process parameters to establish the quality attributes of DPPH radical scavenging activity, total phenolic content, and total flavonoid content of apple (Malus domestica) honey using response surface methodology,” Int. J. Food Prop., vol. 19, no. 8, pp. 1738–1748, 2016, doi: 10.1080/10942912.2015.1107733.

O. M. Okawam M, Kinjo J, Nohara T, “Atividade de eliminação do radical DPPH (1,1-difenil-2-picrilhidrazil) de flavonóides obtidos de algumas plantas medicinais.,” Biol. Pharm. Bull., vol. 24, no. 10, pp. 1202–1205, 2001.

A. M. Shraim, T. A. Ahmed, M. M. Rahman, and Y. M. Hijji, “Determination of total flavonoid content by aluminum chloride assay: A critical evaluation,” Lwt, vol. 150, no. June, p. 111932, 2021, doi: 10.1016/j.lwt.2021.111932.

F. G. da S. Oliveira et al., “Photoprotective activity and HPLC-MS-ESI-IT profile of flavonoids from the barks of Hymenaea martiana Hayne (Fabaceae): development of topical formulations containing the hydroalcoholic extract,” Biotechnol. & Biotechnol. Equip., vol. 35, no. 1, pp. 504–516, 2021.

D. K. K. Dwi Saryanti, “Optimasi Formula Sediaan Krim M/A Dari Ekstrak Kulit Pisang Kepok (Musa Acuminata L.). Departemen Teknologi Farmasi,” J. Ris. Kefarmasian Indones., vol. 1, no. 3, p. 19, 2019.




DOI: https://doi.org/10.37311/ijpe.v4i3.28181

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