Uji Aktivitas Antioksidan Minyak Argan (Argania spinosa L.) Dalam Bentuk Sediaan Mikroemulsi

Nur Ain Thomas, Juliyanty Akuba, Mohammad Adam Mustapa, Adiva Sidangoli

Abstract


Argan oil (Argania spinosa L.) is an oil that has benefits as a natural antioxidant which is good for health. Microemulsion is a thermodynamically stable delivery system;  transparent;  has a small globule size and consists of a mixture of oil, water, surfactant and co-surfactant which has great potential in delivering dermal active substances by increasing transdermal permeability in topical drug delivery. This research aimed to formulate argan (Argania spinosa L.) oil into microemulsion dosage form and determine the antioxidant activity using DPPH method. The study began with the optimization of base into several concentration variations of surfactant and co-surfactant. The formula made into 7 namely F1, F2, F3, F4, F5, F6 and F7, evaluated using organoleptic test and centrifugation test. The results showed the base of F6 which had the clear, transparent physical appearance and no separation occured. The F6 base was then made into microemulsion dosage with 3 concentration variations of argan (Argania spinosa L.) oil, namely F1 (0.5%), F2 (1%) and F3 (1.5%). The 3 formulas were tested for the physical stability which included pH test, viscosity test, centrifugation test and freeze-thaw test.  The testing of antioxidant activity carried out by in vitro using the DPPH method which then calculated for the IC50 value on t0 and t28. From the results of the physical stability test at room temperature and freeze-thaw, the 3 formulas met the organoleptic, pH and centrifugation tests. The antioxidant activity values of IC50 were F1 (t0 = 291.14 g/mL; t28 = 230.43 g/mL), F2 (t0 = 89.02 g/mL; t28 = 129.01 g/mL), F3 (t0 = 81.07 g/mL; t28 = 116.30 g/mL). The statistical test result of T test showed the p value = 0,896, (more than 0.05), which indicated that there was no significance difference in the antioxidant activity result between the 3 formulas on t0and t28.

Keywords


Antioxidant, Microemulsion, Argan Oil, Argania spinosa L.

Full Text:

PDF

References


Yadav, A. Kumari, R., Yadav, A., Mishra, J. P., Srivatva, S. & Prabha, S. (2016). Antioxidants and its functions in human body - A Review. Research in Environment and Life Sciences. Vol 9

Guillaume, D. dan Charrour, Z. (2011). Argan Oil. Medicine Review. Vol 16

Kamal, R., Kharbach, M., Imig, J. D., Eljmeli, M., Doukkali, Z., Mrabti, H. N., Elmsellem, H., Bouklouze, A., Cherrah, Y., Alaoui, K., (2017). Antioxidant activities, total polyphenolic compounds and HPLC/DAD/MS phenolic profile of argan oil derived from two different methods of extractions. Journal of Materials and Environmental Sciences. Vol 8

Chansakaow, S. Sirisa-Ard, P. dan Khonkarn, R. (2017). Preparation, Characterization And Antioxidant Activity Of Xanthone-Loaded Making (Hodgsonia Heteroclita) Microemulsions. International Journal Of Pharmacy And Pharmaceutical Sciences. Vol 9

Kumar, K. S., Dhachinamoorthi, D., Saravanan, R., Gopal, U. K. dan Shanmugam, V. Microemulsions As Carrier For Novel Drug Delivery: A Review. International Journal of Pharmaceutical Sciences Review and Research. Vol 10

Rowe, R.C., Paul J. Sheskey , dan Marian E Quinn. 2009. Handbook Of

Pharmaceutical Excipients. Sixth Edition. Pharmaceutical Press: London

Sulastri, E., Oktaviani, C. dan Yusriadi. 2015. Formulasi Mikroemulsi Ekstrak Bawang Hutan dan Uji Aktivitas Antioksidan. Jurnal Pharmascience. Vol 2

Martin, A., James, S. dan Arthur, C. 1993. Farmasi Fisik: Dasar-dasar Farmasi Fisik dalam Ilmu Farmasetik. UI Press: Jakarta

Pamudji, J. S., Darijanto, S. T. dan Rosa, S. 2012. Formulasi dan Evaluasi Mikroemulsi Minyak dalam Air Betametason 17-Valerat. Acta Pharmaceutica Indonesia. Vol 38

Hasrawati, A., Hasyim, N. dan Irsyad, N. A. 2012. Pengembangan Formulasi Mikroemulsi Minyak Sereh (Cymbopogon nardus) Menggunakan Emulgator Surfaktan Nonionik. Jurnal Fitofarmaka Indonesia. Vol. 3 No.1

Lachman, L. Lieberman, H. A. dan Kanig, J. L. 1976. The Theory and Practice of Industrial Pharmacy. Lea and Febiger. Philadelphia

Vadas, E. B. 2000. Stability of Pharmaceutical Products, dalam Gennaro, A. R.

Remington : The Sciences and Practice of Pharmacy, 20 th edition Jilid I. Philladelphia College of Pharmacy and Sciences

Danimayostu, A. A., Shofiana, N. M. dan Permatasari, D. 2017. Pengaruh Penggunaan Pati Kentang (Solanum tuberosum) Termodifikasi AsetilasiOksidasi sebagai Gelling agent terhadap Stabilitas Gel Natrium Diklofenak. Pharmaceutical Journal Of Indonesia. Vol 3(1)

Sinko, P. J. 2006. Martin’s Physical Pharmacy and Pharmaceutical Sciens Fifth Edition. Lippicott Wiliams & Wilkins. Philadelphia

Fitriani, E. W., Imelda, E., Kornelis, C. dan Avanti, C. 2016. Karakterisasi dan Stabilitas Fisik Mikroemulsi Tipe A/M dengan Berbagai Fase Minyak. Pharm Sci Res. Vol 3

El-Abbassi, A., Neves, M. A., Kobayashi, I., Hafidi, A. dan Nakajima, M., 2013. Preparation and characterization of highly stable monodisperse argan oil-in-water emulsions using microchannel emulsification. Europe Journal Lipid Science Technology. Vol 115

Badarinath, A., Rao, K., Chetty, CS., Ramkanth, S., Rajan, T. dan Gnanaprakash, K. 2010. A Review on In-vitro Antioxidant Methods : Comparisons, Correlations, and Considerations. International Journal of PharmTech Research.




DOI: https://doi.org/10.37311/ijpe.v1i1.9951

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Indonesian Journal of Pharmaceutical Education