Analisis Toksisitas Ekstrak Minyak Sacha Inchi (Plukenetia volubilis)

Finny Nurcita Ningrum Patuti, Yuszda K. Salimi, Najmah Najmah, Astin Lukum, Ahmad Kadir Kilo

Abstract


Penelitian ini bertujuan untuk mengevaluasi tingkat toksisitas ekstrak minyak biji Sacha Inchi (Plukenetia volubilis) menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan larva Artemia salina Leach sebagai organisme uji. Sampel minyak diekstraksi melalui dua metode berbeda, yaitu press hidrolik dan sokletasi. Uji dilakukan pada empat variasi konsentrasi, yaitu 1.250 ppm, 2.500 ppm, 5.000 ppm, dan 10.000 ppm, dengan tiga kali pengulangan untuk masing-masing sampel. Hasil pengujian menunjukkan bahwa persentase mortalitas larva meningkat seiring kenaikan konsentrasi. Pada minyak sacha inchi hasil press hidrolik, mortalitas mencapai 46,7% pada konsentrasi 10.000 ppm, 40,0% pada 5.000 ppm, 10,0% pada 2.500 ppm, dan 0% pada 1.250 ppm. Sementara itu, pada minyak sacha inchi hasil sokletasi, mortalitas larva sebesar 46,7% pada 10.000 ppm, 20,0% pada 5.000 ppm, 13,3% pada 2.500 ppm, dan 0% pada 1.250 ppm. Nilai LC₅₀ diperoleh sebesar 15,249 mg/mL untuk minyak press hidrolik dan 18,532 mg/mL untuk minyak sokletasi. Berdasarkan kriteria toksisitas, kedua nilai tersebut termasuk kategori non-toksik karena memiliki LC₅₀ > 1.000 µg/mL. Hasil ini menunjukkan bahwa minyak biji Sacha Inchi aman digunakan pada uji pendahuluan toksisitas akut berbasis BSLT, serta sejalan dengan karakteristik minyak nabati edible lainnya.

Keywords


BSLT; toxicity; LC₅₀; Artemia salina; Sacha Inchi oil

References


Banti, C. N., & Hadjikakou, S. K. (2021). Evaluation of toxicity with brine shrimp assay. Bio-Protocol, 11(2), 6–12. https://doi.org/10.21769/BioProtoc.3895

Cansian, R. L., Vanin, A. B., Orlando, T., Piazza, S. P., Puton, B. M. S., Cardoso, R. I., Gonçalves, I. L., Honaiser, T. C., Paroul, N., & Oliveira, D. (2017). Toxicidade do óleo essencial de cravo e seu éster acetato de eugenila contra artemia salina. Brazilian Journal of Biology, 77(1), 155–161. https://doi.org/10.1590/1519-6984.12215

Hanafi, H., Irawan, C., Sirait, S., Sulistiawaty, L., & Setyawati, S. (2020). Toxicity Test with BSLT (Brine Shrimp Lethality Test) Method on Methanol, Ethyl Acetate Extract, Hexane on Seeds and Rind of Matoa extract (Pometia pinnata). Oriental Journal Of Chemistry, 36, 1143–1147. https://doi.org/10.13005/ojc/360618

Konan, A. M. L., Golly, K. J., Kra, A. K. M., Adima, A. A., & Lohoues, E. E. C. (2022). Phytochemical Screening and Toxicity Assessment of <>Imperata cylindrica</i> (L.) P. Beauv. (Poaceae) Raw Extracts with Brine Shrimp (<i>Artemia salina</i>) Lethality Assay. Journal of Biosciences and Medicines, 10(08), 153–171. https://doi.org/10.4236/jbm.2022.108014

Kong, S., Keang, T., Bunthan, M., Say, M., Nat, Y., Tan, C. P., & Tan, R. (2023). Hydraulic Cold-Pressed Extraction of Sacha Inchi Seeds: Oil Yield and Its Physicochemical Properties. ChemEngineering, 7(4). https://doi.org/10.3390/chemengineering7040069

Lu, W. C., Chiu, C. S., Chan, Y. J., Mulio, A. T., & Li, P. H. (2025). New perspectives on different Sacha inchi seed oil extractions and its applications in the food and cosmetic industries. Critical Reviews in Food Science and Nutrition, 65(3), 475–493. https://doi.org/10.1080/10408398.2023.2276882

Meyer, B. N., & al., et. (1982). Metode BSLT (Brine Shrimp Lethality Test).

Pohan, D. J., Marantuan, R. S., & Djojosaputro, M. (2023). Toxicity Test of Strong Drug Using the BSLT (Brine Shrimp Lethality Test) Method. International Journal of Health Sciences and Research, 13(2), 203–209. https://doi.org/10.52403/ijhsr.20230228

Roth, S., & Stahl, A. (2016). Jurnal : Entropi. Mechanik Und Wärmelehre, 20(September), 585–623.

Salimi, Y. K., Tungadi, R., Liputo, S. A., Kilo, A. K., Rauf, A. N. P., & Dali, I. B. (2025). Physicochemical Properties, Fatty Acid and Amino Acid Profiles, and Antioxidant Activity of Sacha Inchi (Plukenetia Volubilis L.) Seed Oil and Press Cake Extracted Using Hydraulic Press and Soxhlet Methods. Trends in Sciences, 22(6), 1–17. https://doi.org/10.48048/tis.2025.9690

Sukkum, C., Lekklar, C., Chongsri, K., Deeying, S., Srisomsap, C., Surapanich, N., Kanjanasingh, P., & Hongthong, S. (2025). Anti-cancer activity and brine shrimp lethality assay of the extracts and isolated compounds from Garcinia schomburgkiana Pierre. Journal of Applied Pharmaceutical Science, 15(3), 241–247. https://doi.org/10.7324/JAPS.2025.209800

Ulfa, D. M., Tulandi, S. M., & Sulistiyo, J. (2024). Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents. Tropical Journal of Natural Product Research, 8(4), 6864–6867. https://doi.org/10.26538/tjnpr/v8i4.16

Utomo, A. D. C., & Wihadi, M. N. K. (2022). Preparation of ZnO/TiO2 Nanocomposite Sensitized Mangosteen Rind (Garcinia mangostana L) Dye for Light Harvesting Efficiency in Solar Cell. Indo. J. Chem. Res., 10(2), 68–73. https://doi.org/10.30598//ijcr.2022.10-ary

Wu, C.-H., Yang, M.-Y., & Wang, C.-J. (2019). Quercetin-3-O-glucuronide inhibits doxorubicin resistance by reducing endoplasmic reticulum stress in hepatocellular carcinoma cells. Journal of Functional Foods, 54, 301–309. https://doi.org/https://doi.org/10.1016/j.jff.2019.01.015




DOI: https://doi.org/10.37905/je.v20i2.34267

Refbacks

  • There are currently no refbacks.


Editorial Office

Department of Chemistry, Universitas Negeri Gorontalo
Jl. Prof. Dr. Ing. B. J. Habibie, Moutong, Tilongkabila, Bone Bolango 96554, Gorontalo, Indonesia

E-mail: [email protected]

+6285341773856 (Call/sms/wa)

Jurnal Entropi (JE) (p-ISSN: 1907-1965| e-ISSN: XXX-XXXX) by Department of Chemistry Universitas Negeri Gorontalo. This work is licensed under a Creative Commons Attribution 4.0 International License. Powered by Public Knowledge Project OJS