Aktivitas Antioksidan Fraksi Etil Asetat pada Bunga Kencana Ungu (Ruallia Tuberosa)

Mohamad Launga, Suleman Duengo, Hendri Iyabu, Weny J.A Musa, Akram La Kilo

Abstract


Purple Ruellia (Ruellia tuberosa) contains active compounds and has been empirically used by the community in traditional medicine. This study aimed to determine the antioxidant activity of the ethyl acetate fraction of purple Ruellia flowers. The research was carried out through several stages including extraction and fractionation of the sample followed by phytochemical screening to identify the classes of secondary metabolites. Compound separation was performed using Thin Layer Chromatography (TLC) and Preparative Thin Layer Chromatography. The isolate obtained from preparative TLC was characterized using UV-Vis spectrophotometer and FT-IR, while the antioxidant activity was tested using the DPPH method. The results showed that the ethanol extract had a yield of 27.77%. Phytochemical screening of the ethanol extract and ethyl acetate fraction revealed the presence of flavonoids and tannins. UV-Vis characterization showed two maximum absorptions at wavelengths of 215 nm with an absorbance of 2.238 and 325 nm with an absorbance of 0.933. FT-IR analysis showed absorptions at 3280.71 cm⁻¹ (OH), 2160.92 cm⁻¹ (C≡C and C≡N), 1058.29 cm⁻¹ (C-O), 976.78 cm⁻¹ (C-H), 795.15 cm⁻¹ (C-H), and 477 cm⁻¹ (M-O). The antioxidant activity test of the ethyl acetate fraction showed an IC₅₀ value of 31.70 ppm. This activity is presumed to be due to flavonoid compounds that act as hydrogen donors in neutralizing DPPH free radicals

Keywords


Purple Ruellia; Ethyl acetate fraction; UV-Vis; FTIR; Antioxidant

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DOI: https://doi.org/10.37905/je.v21i2.41356

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