Aktivitas Penghambatan α-Glucosidase Hidrolisat Kolagen dari Sisik dan Tulang Ikan Lolosi Ekor Kuning (Caesio cuning)

Juwita Sulasrina Lalindu, Netty Ino Ischak, Nurhayati Bialangi

Abstract


This reseach aims to determine the collagen content, amino acid profile and α-Glucosidase enzyme inhibitory activity of collagen hydrolysate derived from scales and bones of yellowtail lolosi fish (Caesio cuning). Collagen extraction was performed by acid soluble collagen method using 0.5 M CH3COOH. The extraction results from fish scales and bones obtained by the ASC method obtained yields of 38 g (bb) and 12.22 g (bk). Analysis of collagen content was carried out by UV-Vis spectrophotometric method and the result was 3.63%. Antidiabetic activity test was conducted through inhibition of α-Glucosidase enzyme using microplate reader with Acarbose as positive control. The results showed that collagen hydrolysate had weak inhibitory activity with the % inhibition value at each variation of collagen hydrolysate concentration with IC50 value obtained from the highest concentration of 23.20% and did not reach IC50. Thus, the scales and bones of Caesio cuning fish have the potential as a source of collagen that is useful in the pharmaceutical and health fields.

Keywords


Collagen; Lolosi fish; Caesio cuning; α-Glucosidase

References


Abdullah, N., Ischak, N. I., Alio, L., Salimi, Y. K., & Aman, L. O. (2025). Inhibis Enzim α -Glukosidase d an α -Amilase dari Ekstrak Metanol Daun Buhu ( Garuga floribunda Decne ) Sebagai Antidiabetes.

Ata, S. T. W., Yulianty, R., Sami, F. J., & Ramli, N. (2016). Isolasi Kolagen Dari Kulit Dan Tulang Ikan Cakalang (Katsuwonus pelamis). Journal of Pharmaceutical and Medicinal Science, 1(1), 27–30. https://www.jpms-stifa.com/index.php/jpms/article/view/9

Endah Dwijayanti, Munadi, R., & Farnatubun, M. W. (2023). Analisis Proksimat dan Kolagen pada Kulit Ikan Tawassang (Naso thynnoides). Iltek :Jurnal Teknologi, 18(02), 103–107. https://doi.org/10.47398/iltek.v18i02.124

Firno. (2020). Analisis Kandungan Kolagen Ekstrak Tulang Ikan Nila (Oreochromis niloticus) Asal Kabupaten Gowa. Jurnal Farmasi Dan Bahan Alami, 8(1), 94–100.

Harnedy-Rothwell, P. A., Khatib, N., Sharkey, S., Lafferty, R. A., Gite, S., Whooley, J., O’harte, F. P. M., & Fitzgerald, R. J.(2021).Physicochemical, nutritional and in vitro antidiabetic characterisation of blue whiting (Micromesistius poutassou) protein hydrolysates. Marine Drugs, 19(7). https://doi.org/10.3390/md19070383

Ika, A., Nurjanah, & Tati, N. (2016). Characterization of Acid Soluble Collagen from Redbelly Yellowtail Fusilier Fish Skin (Caesio cuning). Jurnal Pengolahan Hasil Perikanan Indonesia, 19(1), 79–93. https://doi.org/10.17844/jphpi.2016.19.1.79

Ischak, N. I., Aman, L. O., Hasan, H., Kilo, A. La, & Asnawi, A. (2023). In silico screening of Andrographis paniculata secondary metabolites as anti-diabetes mellitus through PDE9 inhibition. Research in Pharmaceutical Sciences, 18(1), 100–111.

Kumar Khatri, D., & Ramesh Juvekar, A. (2015). Α-Glucosidase and Α-Amylase Inhibitory Activity of Indigofera Cordifolia Seeds and Leaves Extract. International Journal Of Pharmacy and Pharmaceutical Sciences, 6(11), 152–155.

Kumar, L. V., Shakila, R. J., & Jeyasekaran, G. (2019). In vitro anti-cancer, anti-diabetic, anti-inflammation and wound healing properties of collagen peptides derived from unicorn leatherjacket (Aluterus monoceros) at different hydrolysis. Turkish Journal of Fisheries and Aquatic Sciences, 19(7), 551–560.

Lee, D. S., & Lee, S. H. (2001). Genistein, a soy isoflavone, is a potent α-glucosidase inhibitor. FEBS Letters, 501(1), 84–86. https://doi.org/10.1016/S0014-5793(01)02631-X

Lubis, E. K., Sinaga, T. Y., & Susiana, S. (2021). Inventarisasi Ikan Demersal dan Ikan Pelagis yang Didaratkan di PPI Kijang Kecamatan Bintan Timur Kabupaten Bintan. Jurnal Akuatiklestari, 4(2), 47–57.

Meila, O., & Noraini, N. (2017). Uji Aktivitas Antidiabetes dari Ekstrak Metanol Buah Kiwi (Actinidia deliciosa) melalui Penghambatan Aktivitas α-Glukosidase. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 3(2), 132–137.

Nur, S., Wierson, Y., Sami, F. J., Megawati, Gani, S. A., Aisyah, A. N., Yulia, & Marwati. (2021). Characterization, antioxidant and α-glucosidase inhibitory activity of collagen hydrolysate from lamuru (caranx ignobilis) fishbone. Sains Malaysiana, 50(8), 2329–2341. https://doi.org/10.17576/jsm-2021-5008-16

Nurhikma, N., Riviani, R., Wulandari, D. A., & Mirsa, M. (2022). Komposisi Kimia dan Aktivitas Kerang Balelo (Conomurex sp.) dalam Menghambat Enzim α-Glukosidase. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 504–511.

Nursa, G., Fauzi, Y., Habibi, J., Studi, P., Masyarakat, K., & Kesehatan, I. (2022). Faktor-Faktor Yang Mempengaruhi Kejadian Diabetes Melitus Di Puskesmas Bintuhan Kabupaten Kaur Tahun 2022 Factors Affecting The Event Diabetes Mellitus In Bintuhan Puskesmas Kaur District Year 2022. Journal Hygea Public Health, 1(1), 1–6.

Pratama, Y., Sarjono, P. R., & Mulyani, N. S. (2015). Skrining Metabolit Sekunder Bakteri Endofit yang Berfungsi sebagai Antidiabetes dari Daun Mimba (Azadirachta Indica). Jurnal Kimia Sains Dan Aplikasi, 18(2), 73–78. https://doi.org/10.14710/jksa.18.2.73-78

Rahmawan Kusa, S., Silvana Naiu, A., Yusuf Universitas Negeri Gorontalo Jl Jend Sudirman No, N., Tim, D., & Kota Tengah, K. (2022). Karakteristik Kolagen Kuit Ikan Tuna Sirip Kuning (Thunnus albacares) Pada Waktu Hidro-Ekstraksi Berbeda Dan Potensinya Dalam Bentuk Sediaan Nanokolagen. Media Teknologi Hasil Perikanan, 10(2), 107–116. https://doi.org/10.35800/mthp.10.2.2022.41716

Robert, M. ., W.Rodwell, V., David, B., M.Botham, K., P., A. W., & J.Petter, K. (2009). Harper’s Illustrated Biochemistry, 28th Edition. In biochemistry (28th editi, pp. 51–71).

Saras, T. (2023). Kolagen: Fondasi Kesehatan Kulit dan Tubuh. Tiram Media. https://www.google.co.id/books/edition/Kolagen_Fondasi_Kesehatan_Kulit_dan_Tubu/yF3jEAAAQBAJ?hl=id&gbpv=0

Sembiring, T. E. S., Reo, A. R., Onibala, H., Montolalu, R. I., Taher, N., Mentang, F., & Damongilala, L. J. (2020). Ekstraksi Kolagen Tulang Ikan Tuna (Thunnus sp) dengan Asam Klorida. Media Teknologi Hasil Perikanan, 8(3), 107.

Suptijah, P., Indriani, D., & Wardoyo, S. E. (2018). Isolasi Dan Karakterisasi Kolagen Dari Kulit Ikan Patin (Pangasius sp.). Jurnal Sains Natural, 8(1), 8.

Widodo, W. (2017). Monitoring of Patient With Diabetes Mellitus. Jurnal Ilmiah Kedokteran Wijaya Kusuma, 3(2), 55. https://doi.org/10.30742/jikw.v3i2.23




DOI: https://doi.org/10.37905/je.v20i1.32452

Refbacks

  • There are currently no refbacks.


Editorial Office

Department of Chemistry, Universitas Negeri Gorontalo
Jl. Prof. Dr. Ing. B. J. Habibie, Moutong, Tilongkabila, Bone Bolango 96554, Gorontalo, Indonesia

E-mail: [email protected]

+6285341773856 (Call/sms/wa)

Jurnal Entropi (JE) (p-ISSN: 1907-1965| e-ISSN: XXX-XXXX) by Department of Chemistry Universitas Negeri Gorontalo. This work is licensed under a Creative Commons Attribution 4.0 International License. Powered by Public Knowledge Project OJS