IDENTIFIKASI SENYAWA ALKALOID PADA DAUN BIDARA ARAB (Ziziphus spina-christi L.) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS

A. Mu’thi Andy Suryadi, Mohammad Adam Mustapa, Nur Khofifah Zahrah

Abstract


Christ's torn jujube (Ziziphus spina-christi L.) is a medicinal plant that has many benefits for curing or treating diarrhea, fever, and cancer. One of the compounds that play role is an alkaloid. This study was generated to identify the alkaloid compounds in the leaves of the Christ’s jujube torn using the UV-Vis spectrophotometry method. Christ's torn jujube leaves were macerated using 3 types of solvents with different polarity levels i.e., n-hexane, ethyl acetate, and methanol. Furthermore, phytochemical screening of alkaloid compounds was carried out. A Christ jujube leaf sample that was positive for alkaloids was found in the ethyl acetate extract; then identified using thin layer chromatography with eluent n-hexane: ethyl acetate (8:2), yielding 4 points with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. Furthermore, phytochemical screening of alkaloid compounds was carried out. Christ' torn jujube which were positive for alkaloids present in the ethyl acetate extract were identified using thin layer chromatography with n-hexane eluent: ethyl acetate (8:2), yielding four spots with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. The four spots were identified using UV-Vis spectrophotometry, obtained a wavelength of 248 ; 268 ; 268 ; and 255. Based on the identification results on the leaf extract, each stain was suspected to contain cantin-6-one alkaloid compounds.

Keywords


Christ’s Torn Jujube (Ziziphus spina-christi L.), Thin Layer Chromatography, UV-Vis Spectrophotometry

References


Bonofiglio D., Plastina P., Vizza D. 2012. Identification Of Bioactive Constituents Of Ziziphus Jujube Fruit Extracts Exerting Antiproliferative And Apoptotic Effects In Human Breast Cancer Cells. J Ethnopharmacol. 325-338.

Lestari T., Ruswanto., Garna IM., Tuslinah L., Richa M., Nofianti T. 2018. Kuersetin : Penghambat Uridin 5-Monofosfat Sintase Sebagai Kandidat Antikanker. Alchemy Jurnal Penelitian Kimia.

Mauludiyah, Elsya Nurul., Fitrianti Darusman., Gita Cahya Eka Darma. 2020. Skrining Fitokimia Senyawa Metabolit Sekunder Dari Simplisia Dan Ekstrak Air Daun Bidara Arab (Ziziphus Spina-Christi L.). Bandung : Universitas Islam Bandung. 1084-1089.

Nururrahmah Hammado., Ilmiati Illing. 2013. Identifikasi Senyawa Bahan Aktif Alkaloid Pada Tanaman Lahuna (Eupatorium odoratum). Makassar : Universitas Cokroaminoto Palopo.

Ningrum R., Purwanti E., Sukarsono. 2016. Identifikasi Senyawa Alkaloid Dari Batang Karamunting (Rhodomyrtus Tomentosa) Sebagai Bahan Ajar Biologi Untuk SMA Kelas X. Malang : Universitas Muhammadiyah Malang. 231-236.

Nessel, F. M. 2008. Isolasi Alkaloid Utama dari Tumbuhan Lerchea interrupta Korth. Jambi : Balai Pengawasan Obat dan Makanan.

Feriyanto, Y. 2013. Pengambilan Minyak Atsiri dari Daun dan Batang Sereh Wangi (Cymbopogonwinterianus) Menggunakan Metode Distilasi Uap Air dengan Pemanasan microwave. Surabaya : Institut Teknologi 10 November.

Departemen Kesehatan Republik Indonesia. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan.

Muksin Maulana. 2018. Profil Kromatografi Lapis Tipis (KLT) Ekstrak Daun Bidara Arab (Ziziphus spina-christi l.) Berdasarkan Variasi Pelarut. Malang : Universitas Islam Negeri Maulana Malik Ibrahim.

Christina Astutiningsih., Frida Nuzulia., Agus Suprijono. 2012. Isolasi Dan Identifikasi Senyawaalkaloid Buah Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl) Secara Spektrofotometri Uv-Vis Dan Ir Serta Uji Toksisitas Akut Terhadap Larva Artemia salina Leach. Semarang : Sekolah Tinggi Ilmu Farmasi “Yayasan Pharmasi”.

Harborne, J. B. 1996. Metode Fitokimia. Bandung : Penerbit ITB.

Hostettmann K., M Hostettman, MD., Marston A. 1995. Cara kromatografi preparatif. Penggunan pada Isolasi Senyawa Alam. Bandung : Institut Teknologi Bandung.

Gasparic, J., Churacek, J. 1978. Laboratory Handbook of Paper and. Thin Layer Chromatography. New York : John Wiley and Sons.

Gandjar, I. G., Rohman, A. 2007. Kimia Farmasi Analisis. Yogyakarta : Pustaka Pelajar.

Worsfold, P., Townshend, A., Poole, C., Miro, M. 2019. Encyclopedia of Analytical Science (3rd Ed.). Belanda : Amsterdam.




DOI: https://doi.org/10.37311/ijpe.v2i1.11687

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Indonesian Journal of Pharmaceutical Education