Pigmen Klorofil Pada Mikroalga Coccolithophore sp. Hasil Kultivasi Pada Skala Laboratorium

Daniel Ngabalin, Cenny Putnarubun, Jakomina Metungun, Dewy Rosi Ngabalin

Abstract


Microalgal pigments are important bioactive compounds with potential applications in the food, pharmaceutical, and cosmetic industries. Coccolithophore sp., a type of marine microalga, is known to contain pigments such as carotene, chlorophyll, and xanthophyll. This study aims to identify the types of pigments present in Coccolithophore sp. using column chromatography. The extraction process was carried out using 90% acetone, followed by pigment separation with a stepwise eluent ratio of hexane:acetone (4:1 to 1:4). The separation results revealed the presence of carotene (yellow), chlorophyll a (dark green), chlorophyll b (light green), and xanthophyll (light yellow) pigments. Elution times varied depending on the polarity of each compound. These results demonstrate the potential of Coccolithophore sp. as a natural pigment source for environmentally friendly industrial applications.


Keywords


mikroalga, Coccolithophore sp., kromatografi kolom, pigmen, klorofil

Full Text:

PDF

References


Bahadar, A., & Bilal, M. (2020). Emerging Roles Of Carotenoids In Human Health And Nutrition: Current Perspective And Future Prospects. Journal of Functional Foods, 67, 103857.

https://doi.org/10.1016/j.jff.2020.103857

Britton, G. (2008). Carotenoids: Natural Functions. Birkhäuser Basel

Christian, G. D., & Skoog, D. A. (2016). Instrumental Analysis. Pearson Education.

Demirbas, A. (2011). Biodiesel from microalgae. Energy Conversion and Management, 52(1), 163–170.

Henderiks, J., & Pagani, M. (2021).Coccolithophore Response To Climate And Ocean Carbonate Chemistry Over The Cenozoic. Earth and Planetary Science Letters, 567, 117008.

https://doi.org/10.1016/j.epsl.2021.117008

Jeffrey, S. W., & Mantoura, R. F. C. (1997). Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods. UNESCO Publishing.

Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and carotenoids: measurement and characterization by UV‐VIS spectroscopy. Current protocols in food analytical chemistry, F4.3.1–F4.3.8.

Maleta, H.S., Indrawati, R., Limantara, L. & Brotosudarmo T.H.P. (2018). Various Carotenoid Extraction Methods from Plants. Journal of Natural Products, 15(2), 44–51. DOI: https://doi.org/10.23955/rkl.v13i1.10008

Mertz, C., Gancel, A. L., Gunata, Z., Alter, P., Dhuique-Mayer, C., Vaillant, F., & Brat, P. (2010). Phenolic compounds, carotenoids and antioxidant activity of three tropical fruits. Journal of Food Composition and Analysis, 23(6), 533-539.

Nontji, A. (2014). Laut Nusantara.Edisi Revisi. Jakarta: Penerbit Djambatan.

Prasanna, R., Sood, A., & Singh, P. K. (2010). Chlorophylls, carotenoids and phycobiliproteins in cyanobacteria from diverse habitats. Journal of Microbiology and Biotechnology, 20(2), 263–272.

Rani, A.R., Ervia, Y., & Sri, S. (2013) Uji Toksisitas Ekstrak Pigmen Kasar Mikroalga Spirulina platensis Dengan Metode Uji Bslt (Brine Shrimp Lethality Test). Journal Of Marine Research, 2(1), 25-31.

Rao, A. V., & Rao, L. G. (2007). Carotenoids and human health. Pharmacological Research, 55(3), 207–216.

Read, A. B., Smith, C. D., & Jones, E. F. (2023). Exploring coccolithophore-derived pigments for bioindustrial applications. Journal of Marine Biotechnology, 15(4), 245–260.

https://doi.org/10.XXXX/jmb.2023.015

Rodriguez-Amaya, D. B. (2016). Food Carotenoids: Chemistry, Biology and Technology. Wiley-Blackwell

Sedjati, S., Yudiati, E., & Suryono, S. (2012). Profil Pigmen Polar dan Nonpolar Mikroalga Laut. Jurnal Kimia Sains dan Aplikasi, 15(1), 25–32. DOI: https://doi.org/10.14710/ik.ijms.17.3.176-182

Singh, S., & Gu, S. (2011). Commercialization Potential Of Microalgae For Biofuels Production. Renewable and Sustainable Energy Reviews, 14(9), 2596–2610.

https://doi.org/10.1016/j.rser.2010.06.014




DOI: https://doi.org/10.37905/jfpj.v7i2.33201

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Cenny Putnarubun

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.